• Sinopodophyllum hexandrum is an alpine medicinal plant that produces the anticancer compound podophyllotoxin (PPT). Although a positive relationship between PPT content and altitude has been proved and low temperature enhances plant growth and PPT accumulation has also been revealed, the role of UV radiation in regulating growth and PPT accumulation is still unclear
• In this study, morphophysiological traits, metabolites content and related genes expression were investigated by exposing S. hexandrum seedlings to treatment with UV‐B radiation.
• The results showed that the contents of soluble sugars and flavonoids, and the expression levels of genes involved in glycometabolism (XET and β‐1,3‐glucanase) and flavonoid biosynthesis (PAL,C4H,4CL,CHS1 and DTX41) were enhanced in response to UV‐B compared to CK. Moreover, genes involved in stress tolerance (MYB, WRKY,APX3 and EX2) were also upregulated in response to UV‐B radiation. Although the whole plant biomass exhibited slightly increased values that depended largely on root development, the contents of chlorophyll and PPT and the expression levels of genes involved in photosynthesis (matK, ndhF,rbcL and ycf5) and PPT biosynthesis (C3H,CCoAMT,CCR,CAD, DPO, PLR,SDH, CPY719A23,OMT3,CYP71CU1,OMT1and 2‐ODD) were significantly decreased in response to UV‐B compared to CK.
• It can be concluded that UV‐B radiation promotes soluble sugars and flavonoids accumulation, but inhibits PPT biosynthesis in S. hexandrum.

中文翻译：

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UV-B辐射对六味木生长，黄酮和鬼臼毒素累积及相关基因表达的影响

• 鬼臼（Sinopodophyllum hexandrum）是一种高山药用植物，可产生抗癌化合物鬼臼毒素（PPT）。尽管已经证明了PPT含量与海拔高度之间呈正相关关系，并且低温促进了植物的生长和PPT的积累，但紫外线在调节生长和PPT积累中的作用仍不清楚
• 在这项研究中，通过将六瓣链霉菌幼苗暴露于UV-B辐射下，研究了形态生理特性，代谢产物含量和相关基因的表达。
• 结果表明，可溶性糖和类黄酮的含量以及参与糖代谢的基因（XET和β-1,3-葡聚糖酶）和类黄酮的生物合成（PAL，C4H，4CL，CHS1和DTX41）的表达水平均得到提高。与CK比较 此外，与胁迫耐性有关的基因（MYB，WRKY，APX3和EX2）也响应UV-B辐射而上调。尽管整个植物的生物量显示出略有增加的值，这在很大程度上取决于根的发育，但叶绿素和PPT的含量以及参与光合作用的基因（matK，ndhF，rbcL和ycf5）和PPT生物合成的基因表达水平（C3H，CCoAMT，CCR，CAD，DPO，PLR，SDH，CPY719A23，OMT3，CYP71CU1，OMT1和2-ODD与CK相比，对UV-B的响应显着降低）。
• 可以得出结论，UV-B辐射促进可溶性糖和类黄酮的积累，但会抑制六六糖中PPT的生物合成。