Biscutella auriculata L. is a plant that belongs to the Brassicaceae family and it has been found growing in a metal-contaminated area of the San Quíntín mine (Ciudad Real, Spain). The purpose of this work was to evaluate the mechanisms that allow this plant to tolerate high concentrations of copper. Seedlings were grown in a semi-hydroponic system for 15 days under 125 μM of Cu (NO₃)₂. Exposure to copper resulted in growth inhibition and reduction in the photosynthetic parameters. Copper was mainly accumulated in vascular tissue and vacuoles of the roots and only a minor proportion was transferred to the shoot. Biothiol analysis showed a greater enhancement of reduced glutathione in leaves and increases of phytochelatins (PC2 and PC3) in both leaves and roots. Copper treatment induced oxidative stress, which triggered a response of the enzymatic and non-enzymatic antioxidant mechanisms. The results show that B. auriculata is able to tolerate high metal levels through the activation of specific mechanisms to neutralize the oxidative stress produced and also by metal sequestration through phytochelatins. The preferential accumulation of copper in roots provides clues for further studies on the use of this plant for phytostabilization and environmental recovery purposes in Cu-contaminated areas.

中文翻译：

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Biscutella auriculata L 铜稳态和耐受性的生化基础表征。

Biscutella auriculata L. 是一种属于十字花科的植物，已发现它生长在 San Quíntín 矿（西班牙雷亚尔城）的金属污染区。这项工作的目的是评估使该植物耐受高浓度铜的机制。幼苗在半水培系统中在 125 μM C​​u (NO ₃ ) ₂下生长 15 天. 暴露于铜会导致生长抑制和光合参数降低。铜主要积累在根的维管组织和液泡中，只有一小部分转移到地上部。生物硫醇分析表明，叶片中还原型谷胱甘肽的作用更大，叶片和根中的植物螯合素（PC2 和 PC3）增加。铜处理诱导氧化应激，引发酶促和非酶促抗氧化机制的反应。结果表明，B. auriculata能够通过激活特定机制以中和产生的氧化应激以及通过植物螯合素螯合金属来耐受高金属水平。铜在根中的优先积累为进一步研究将该植物用于铜污染地区的植物稳定和环境恢复目的提供了线索。