当前位置: X-MOL 学术J. Physiol. Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
NK cell-derived exosomes improved lung injury in mouse model of Pseudomonas aeruginosa lung infection
The Journal of Physiological Sciences ( IF 2.3 ) Pub Date : 2020-10-23 , DOI: 10.1186/s12576-020-00776-9
Ruiqi Jia 1 , Kuili Cui 2 , Zhenkui Li 2 , Yuan Gao 2 , Bianfang Zhang 2 , Zhixia Wang 3 , Junwei Cui 2
Affiliation  

Background
Pseudomonas aeruginosa ( PA ) is one of the most common bacteria that causes lung infection in hospital. The aim of our study is to explore the role and action mechanism of NK cells in lung PA infection.

Methods
In this present study, 2.5 × 108 CFU/mouse PA was injected into murine trachea to make lung PA infection mouse model. Anti-asialo GM1 was used to inhibit NK cell. The percentage of NK cells was ensured by flow cytometry, and the M1- and M2-polarized macrophages were determined by flow cytometry, qRT-PCR, and ELISA assay. Besides, H&E staining was performed to ensure the pathological changes in lung tissues. Transmission electron microscopy and western blot were carried out to identify the exosome.

Results
Here, in the mouse model of PA lung infection, NK cell depletion caused M2 polarization of lung macrophage, and exacerbated PA -induced lung injury. Next, our data shown that M2 macrophage polarization was enhanced when the generation of NK cell-derived exosome was blocked in the co-culture system of NK cells and macrophages. Subsequently, we demonstrated that NK cells promoted M1 macrophage polarization both in PA -infected macrophage and the mouse model of PA lung infection, and attenuated lung injury through exosome.

Conclusion
Overall, our data proved that NK cell may improve PA -induced lung injury through promoting M1 lung macrophage polarization by secreting exosome. Our results provide a new idea for the treatment of PA lung infection.



中文翻译:

NK细胞来源的外泌体改善铜绿假单胞菌肺部感染小鼠模型的肺损伤

背景
铜绿假单胞菌 PA )是导致医院肺部感染最常见的细菌之一。我们研究的目的是探讨 NK 细胞在肺 PA 感染中的作用和作用机制 。

方法
在本研究中,将 2.5 × 10 8  CFU/小鼠 PA 注射到小鼠气管中以制备肺 PA 感染小鼠模型。Anti-asialo GM1用于抑制NK细胞。NK 细胞的百分比由流式细胞术确定,M1 和 M2 极化的巨噬细胞通过流式细胞术、qRT-PCR 和 ELISA 测定确定。此外,进行H&E染色以确保肺组织的病理变化。进行透射电子显微镜和蛋白质印迹以鉴定外泌体。

结果
这里,在 PA 肺部感染的小鼠模型中,NK 细胞耗竭导致肺巨噬细胞 M2 极化,并加剧 PA -诱发的肺损伤。接下来,我们的数据显示,当 NK 细胞和巨噬细胞的共培养系统中 NK 细胞衍生的外泌体的产生被阻断时,M2 巨噬细胞极化增强。随后,我们证明 NK 细胞在 PA 感染的巨噬细胞和 PA 肺部感染的小鼠模型中 促进 M1 巨噬细胞极化 ,并通过外泌体减轻肺损伤。

结论
总的来说,我们的数据证明NK细胞可以通过分泌外泌体促进M1肺巨噬细胞极化来改善 PA 诱导的肺损伤。我们的研究结果为 PA 肺部感染的治疗提供了新思路 。

更新日期:2020-10-23
down
wechat
bug