Curcumin, quercetin and rutin are flavonoids having strong antioxidant potential, individually used in treatment of numerous ailments. The safety assessment of each of them is already established but no toxicological assessment has been done that would guarantee the safe use of these three flavonoids when used as a polyherbal combination (PHC). The aim of this study to evaluate the possible toxicological effect of polyherbal combination of these three flavonoids in female Swiss albino mice. In acute toxicity study, the oral dose of poly herbal combination was administered to four groups stepwise in single dose and general behaviour, adverse effects and mortality were determined up to 14 days and compared to normal group. In sub-acute study, the tested poly herbal combination was administered orally for 28 days to the four experimental groups and their body weight was measured each alternate day from the first day of dosing. On 29th day the final body weight was recorded and euthanized by using thiopentone sodium, blood was collected and later haematological, lipid profile, biochemical parameter was evaluated and compared to normal group. In acute toxicity study, no abnormal general behaviour, adverse effects were reported. No significant changes were reported in body weight, haematological, lipid profile, biochemical parameter in sub-acute toxicity study. No mortality was reported in both the study. Histopathological examination revealed no alterations in clinical signs or organ weight at any dose. The result concludes that the oral administration of Polyherbal combination did not produce any significant toxic effect in swiss albino mice. Hence, the Polyherbal combination can be utilized safely for therapeutic use.

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姜黄素，槲皮素和芦丁多聚醚组合的毒性和安全性的探索性分析

姜黄素，槲皮素和芦丁是具有很强抗氧化能力的黄酮类化合物，分别用于治疗多种疾病。已经对它们中的每一种进行了安全性评估，但是还没有进行毒理学评估来保证将这三种类黄酮用作聚草药组合（PHC）时可以安全使用。这项研究的目的是评估这三种类黄酮的多草药组合对雌性瑞士白化病小鼠的可能的毒理作用。在急性毒性研究中，将多草药组合的口服剂量按单剂量和一般行为分四个阶段逐步给药，确定不良反应和死亡率直至14天，并与正常组进行比较。在亚急性研究中 将测试的多草药组合口服给药至四个实验组28天，并从给药第一天起每隔一天测量一次它们的体重。在第29天，记录最终体重并通过使用硫代戊酮钠对安乐死进行安乐死，收集血液，随后评估血液学，脂质谱，生化参数并将其与正常组进行比较。在急性毒性研究中，未报告异常的一般行为，不良反应。在亚急性毒性研究中，体重，血液学，脂质分布，生化参数均无明显变化。两项研究均未报告死亡率。组织病理学检查显示任何剂量的临床体征或器官重量均无变化。该结果得出结论，口服聚草醚组合对瑞士白化病小鼠没有产生任何明显的毒性作用。因此，多草药组合可以安全地用于治疗用途。