l-Cyteine is a form of amino acid found in human body. Retaining the exact quantity of l-Cyteine is important for better functioning of the body. A novel hybrid TiO₂ nanostructure (H-TNTs) was prepared on both sides of Ti sheet using the first-step anodization in used (residual) ethylene glycol (EG) based electrolyte. The H-TNTs was explored as an enzyme-free electrochemical biosensor for the detection of l-Cysteine (L-Cyst) and hydrogen peroxide (H₂O₂). Structural analysis revealed that annealed H-TNTs was anatase with uniform tube morphology and narrow pore size distribution of the top thin nanoporous layer. Electrochemical measurements demonstrated excellent electrocatalytic activities of H-TNTs with sensitivity and rapid response of L-Cyst (0.9914 μA mM⁻¹ cm⁻², <2 s) and H₂O₂ (85.3 μA mM⁻¹ cm^−2, < 5 s) respectively. The DFT analysis described that TNT has greater affinity towards L-Cyst and H₂O₂ with stronger binding distances after the adsorption. The higher negative E_ads values suggesting the stable and chemisorptions nature of the H-TNTs. The density of states (DOS) results show that E_gap of TNT was significantly reduced after both molecules adsorption. The fabricated electrochemical biosensor exhibited decent stability, excellent reproducibility, and good resistance to interfering molecules showing great potential, as a unique, non-enzymatic electrochemical sensor for future medical applications.

1- Cyteine是人体中一种氨基酸。保留1- Cyteine的确切数量对于身体更好的功能很重要。使用第一步（残留的）基于乙二醇（EG）的电解质进行第一步阳极氧化，在Ti片的两面上制备了一种新型的TiO ₂纳米杂化混合结构（H-TNTs）。该H-TNTS被探索作为用于检测的不含酶的电化学生物传感器升-半胱氨酸（L-囊肿）和过氧化氢（H ₂ ö ₂）。结构分析表明，退火后的H-TNTs为锐钛矿型，其管形均匀，顶部纳米多孔层的孔径分布窄。电化学测量表明，H-TNT具有出色的电催化活性，灵敏度和L-Cyst（0.9914μAmM ^-1  cm ^-2，<2 s）和H ₂ O ₂（85.3μAmM ^-1  cm ^-2， <5 s）。DFT分析表明，TNT对L-Cyst和H ₂ O ₂具有更高的亲和力，吸附后的结合距离更强。较高的负面E_广告值表明H-TNT的稳定和化学吸附性质。状态密度（DOS）结果表明，两种分子吸附后，TNT的E_间隙均显着降低。制成的电化学生物传感器具有独特的稳定性，出色的可重复性，并且对显示出巨大潜力的干扰分子具有良好的抵抗力，是一种独特的非酶电化学传感器，可用于未来的医学应用。