Background

As the survival rate of premature infants increases, the incidence of bronchopulmonary dysplasia (BPD), a chronic complication of premature infants, is also higher than before. The pathogenesis of BPD is complicated, and immune imbalance and inflammatory response may play important roles in it.

Objective

To investigate the correlation between lymphocyte subsets in peripheral blood, especially γδ-T cells, and BPD of preterm infants.

Materials and method

The study was carried out with the peripheral blood of premature infants (GA < 32 weeks, BW < 1500 g), which were collected at 24 h or 3–4 weeks after birth. The infants were divided into non-BPD groups and BPD groups that were classified as mild or moderate and severe in preterm infants based on the magnitude of respiratory support at 28 days age and 36 weeks postmenstrual age. The γδ-T, CD3+, CD4+, CD8+ and total lymphocyte subsets in peripheral blood were detected by flow cytometry.

Results

The percentages of T lymphocyte subsets in peripheral blood were not different between BPD and non-BPD within 24 h after birth. And no significant difference was found in T lymphocyte subsets among neonates with BPD of different severities. However, the infants who developed BPD had a significant increase in γδ-T cells compared to non-BPD ones within 3–4 weeks after birth.

Conclusions

It seems that γδ-T cells in peripheral blood are correlated with BPD. However, the causality of BPD and various lymphocytes remains unclear, which need to be further studied.

中文翻译：

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γδ-T细胞与早产儿支气管肺发育不良的关系

背景

随着早产儿存活率的提高，早产儿慢性并发症支气管肺发育不良（BPD）的发病率也比以前高。BPD的发病机制复杂，免疫失衡和炎症反应可能在其中起重要作用。

客观的

探讨外周血淋巴细胞亚群特别是γδ-T细胞与早产儿BPD的相关性。

材料与方法

该研究使用早产儿（GA < 32 周，BW < 1500 g）的外周血进行，这些血在出生后 24 小时或 3-4 周收集。根据早产儿 28 天龄和 36 周龄时呼吸支持的程度，将婴儿分为非 BPD 组和 BPD 组，这些组早产儿分为轻度或中度和重度。流式细胞术检测外周血γδ-T、CD3+、CD4+、CD8+和总淋巴细胞亚群。

结果

出生后24小时内BPD和非BPD外周血T淋巴细胞亚群百分比无差异。不同严重程度的BPD新生儿的T淋巴细胞亚群差异无统计学意义。然而，与非 BPD 婴儿相比，患有 BPD 的婴儿在出生后 3-4 周内的 γδ-T 细胞显着增加。

结论

外周血中的γδ-T细胞似乎与BPD相关。但BPD与各种淋巴细胞的因果关系尚不清楚，有待进一步研究。