Natural products have presented potentiality to improve the outcomes of cancer therapies. Impressic acid (E12-1) and acankoreanogein (E13-1), important activity compounds in Acanthopanax trifoliatus (L.) Merr., show widely biological activities. In this study, we isolated E12-1 and E13-1 from Acanthopanax trifoliatus (L.) Merr., and investigated their improvement effect in gemcitabine (GEM) treatment in Panc-1 cells. The results showed that GEM in combination with E12-1 or E13-1 showed stronger inhibition on the growth and induction of apoptosis in Panc-1 cells compared to GEM, E12-1, or E13-1 alone. GEM in combination with E12-1or E13-1 also strongly inhibited cell migration. Mechanistic investigation showed that GEM in combination with E12-1or E13-1 effectively inhibited the activition of nuclear factor kappa-light-chain-enhancer of activated B cells and Stat 3. Overall, GEM in combination with E12-1 or E13-1 might be an effective strategy for the prevention of prostate cancer.

天然产物具有改善癌症治疗结果的潜力。刺五加（L.）Merr。中的重要活性化合物-异酸（E12-1）和acankoreanogein（E13-1）具有广泛的生物活性。在这项研究中，我们从刺五加分离出E12-1和E13-1（L.）Merr。，并研究了它们在Panc-1细胞中对吉西他滨（GEM）治疗的改善作用。结果表明，与单独的GEM，E12-1或E13-1相比，GEM与E12-1或E13-1组合对Panc-1细胞的生长和诱导的凋亡具有更强的抑制作用。GEM与E12-1或E13-1的结合也强烈抑制了细胞迁移。机理研究表明，GEM与E12-1或E13-1结合可有效抑制活化的B细胞和Stat 3的核因子κ轻链增强子的活化。总的来说，GEM与E12-1或E13-1结合可能是预防前列腺癌的有效策略。