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A Variable Scheduling Maintenance Culture Platform for Mammalian Cells
SLAS Technology: Translating Life Sciences Innovation ( IF 2.7 ) Pub Date : 2020-12-03 , DOI: 10.1177/2472630320972109
Koji Ochiai 1 , Naohiro Motozawa 2, 3 , Motoki Terada 2 , Takaaki Horinouchi 1, 4 , Tomohiro Masuda 2 , Taku Kudo 5 , Motohisa Kamei 5 , Akitaka Tsujikawa 3 , Kenji Matsukuma 5 , Tohru Natsume 5, 6 , Genki N Kanda 1, 2, 5 , Masayo Takahashi 2, 7 , Koichi Takahashi 1
Affiliation  

Cell culturing is a basic experimental technique in cell biology and medical science. However, culturing high-quality cells with a high degree of reproducibility relies heavily on expert skills and tacit knowledge, and it is not straightforward to scale the production process due to the education bottleneck. Although many automated culture systems have been developed and a few have succeeded in mass production environments, very few robots are permissive of frequent protocol changes, which are often required in basic research environments. LabDroid is a general-purpose humanoid robot with two arms that performs experiments using the same tools as humans. Combining our newly developed AI software with LabDroid, we developed a variable scheduling system that continuously produces subcultures of cell lines without human intervention. The system periodically observes the cells on plates with a microscope, predicts the cell growth curve by processing cell images, and decides the best times for passage. We have succeeded in developing a system that maintains the cultures of two HEK293A cell plates with no human intervention for 192 h.



中文翻译:

哺乳动物细胞的可变调度维护培养平台

细胞培养是细胞生物学和医学科学中的一项基本实验技术。然而,培养具有高度可重复性的高质量细胞在很大程度上依赖于专业技能和隐性知识,并且由于教育瓶颈,规模化生产过程并不简单。尽管已经开发了许多自动化培养系统,并且有一些系统在大规模生产环境中取得了成功,但很少有机器人允许频繁的协议更改,而这在基础研究环境中通常是必需的。LabDroid 是一种通用人形机器人,具有两条手臂,可以使用与人类相同的工具进行实验。将我们新开发的 AI 软件与 LabDroid 相结合,我们开发了一个可变调度系统,可以在没有人工干预的情况下连续生产细胞系的亚培养。系统定期用显微镜观察平板上的细胞,通过处理细胞图像预测细胞生长曲线,并决定最佳传代时间。我们已经成功开发了一个系统,可以在没有人为干预的情况下保持两个 HEK293A 细胞板的培养物 192 小时。

更新日期:2020-12-04
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