Hypertensive disorders are common complications during pregnancy (HDP) with substantial public health impact. Acute and chronic particulate matter (PM) exposure during pregnancy increases the risk of HDP, although the underlying molecular mechanisms remain unclear. Extracellular vesicles (EVs) may be the ideal candidates for mediating the effects of PM exposure in pregnancy as they are released in response to environmental stimuli. The INSIDE project aims to investigate this mechanism in pregnancy outcomes. The study population is enrolled at the Fetal Medicine Unit of Fondazione IRCCS Ca’Granda—Ospedale Maggiore Policlinico at 10–14 weeks of gestation. Exposure to PM₁₀ and PM_2.5 is assessed using the flexible air quality regional model (FARM) and Bayesian geostatistical models. Each woman provides a blood sample for EV analysis and circulating biomarker assessment. Moreover, a subgroup of recruited women (n = 85) is asked to participate in a cardiovascular screening program including a standard clinical evaluation, a non-invasive assessment of right ventricular function, and pulmonary circulation at rest and during exercise. These subjects are also asked to wear a personal particulate sampler, to measure PM₁₀, PM_2.5, and PM₁. The INSIDE study is expected to identify the health impacts of PM exposure on pregnancy outcomes.

中文翻译：

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INSIDE项目：孕妇的个人空气污染暴露，细胞外囊泡信号传导和高血压疾病的发展

高血压疾病是怀孕期间的常见并发症（HDP），对公共健康有重大影响。尽管尚不清楚潜在的分子机制，但怀孕期间暴露于急性和慢性颗粒物（PM）会增加HDP的风险。细胞外囊泡（EVs）可能是介导孕妇PM暴露影响的理想候选物，因为它们是对环境刺激而释放的。INSIDE项目旨在研究这种妊娠结局机制。研究人群在妊娠10-14周时进入IRCCS Ca'Granda-Ospedale Maggiore Policlinico胎儿医学科。暴露于PM ₁₀和PM _2.5使用灵活的空气质量区域模型（FARM）和贝叶斯地统计模型进行评估。每个妇女都提供血液样本用于EV分析和循环生物标志物评估。此外，要求一组新招募的妇女（n = 85）参加心血管筛查计划，包括标准临床评估，右心室功能的非侵入性评估以及休息和运动期间的肺循环。还要求这些受试者佩戴个人颗粒物采样器，以测量PM ₁₀，PM _2.5和PM ₁。预计INSIDE研究将确定PM暴露对妊娠结局的健康影响。