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Inducing different severities of traumatic brain injury in Drosophila using a piezoelectric actuator
Nature Protocols ( IF 14.8 ) Pub Date : 2020-12-04 , DOI: 10.1038/s41596-020-00415-y
Janani Saikumar 1 , Joshua Kim 1 , China N Byrns 2 , Matthew Hemphill 3 , David F Meaney 3, 4 , Nancy M Bonini 1, 2
Affiliation  

Drosophila models have been instrumental in providing insights into molecular mechanisms of neurodegeneration, with wide application to human disease. The brain degeneration associated with traumatic brain injury (TBI) has been modeled in Drosophila using devices that inflict trauma on multiple parts of the fly body, including the head. However, the injuries produced by these models are not specific in location and are inconsistent between individual animals. We have recently developed a device that can be used to inflict controlled head injury to flies, resulting in physiological responses that are remarkably similar to those observed in humans with TBI. This protocol describes the construction, calibration and use of the Drosophila TBI (dTBI) device, a platform that employs a piezoelectric actuator to reproducibly deliver a force in order to briefly compress the fly head against a metal surface. The extent of head compression can be controlled through an electrical circuit, allowing the operator to set different levels of injury. The entire device can be assembled and calibrated in under a week. The device components and the necessary electrical tools are readily available and cost ~$800. The dTBI device can be used to harness the power of Drosophila genetics and perform large-scale genetic or pharmacological screens, using a 7-d post-injury survival curve to identify modifiers of injury.



中文翻译:

使用压电致动器在果蝇中诱导不同严重程度的创伤性脑损伤

果蝇模型有助于深入了解神经变性的分子机制,并广泛应用于人类疾病。与外伤性脑损伤 (TBI) 相关的脑退化已经在果蝇中进行了建模,使用的设备会对蝇体的多个部位(包括头部)造成创伤。然而,这些模型产生的损伤在位置上并不具体,并且在个体动物之间是不一致的。我们最近开发了一种设备,可用于对苍蝇造成可控的头部伤害,从而产生与 TBI 人类观察到的生理反应非常相似的生理反应。该协议描述了果蝇的构造、校准和使用TBI (dTBI) 设备,一个平台,采用压电致动器可重复地传递力,以便将飞行头短暂地压缩在金属表面上。可以通过电路控制头部压缩的程度,允许操作员设置不同程度的伤害。整个设备可以在一周内组装和校准。设备组件和必要的电动工具很容易获得,成本约为 800 美元。dTBI 设备可用于利用果蝇遗传学的力量并进行大规模的遗传或药理学筛选,使用 7 天损伤后生存曲线来识别损伤修饰符。

更新日期:2020-12-04
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