The chitin-assimilating gram-negative bacterium, Lysobacter sp. MK9-1, was isolated from soil and was the source of a glycoside hydrolase family 19-type chitinase (Chi19MK) gene that is 933-bp long and encodes a 311-residue protein. The deduced amino acid sequence of Chi19MK includes a signal peptide, an uncharacterized sequence, a carbohydrate-binding module family 12-type chitin binding domain, and a catalytic domain. The catalytic domain of Chi19MK is approximately 60% similar to those of ChiB from Burkholderia gladioli CHB101, chitinase N (ChiN) from Chitiniphilus shinanonensis SAY3^T, ChiF from Streptomyces coelicolor A3(2), Chi30 from Streptomyces olivaceoviridisis, ChiA from Streptomyces cyaneus SP-27, and ChiC from Streptomyces griseus HUT6037. Chi19MK lacking the signal and uncharacterized sequences (Chi19MKΔNTerm) was expressed in Escherichia coli Rosetta-gami B(DE3), resulting in significant chitinase activity in the soluble fraction. Purified Chi19MKΔNTerm hydrolyzed colloidal chitin and released disaccharide. Furthermore, Chi19MKΔNTerm inhibited hyphal extension in Trichoderma reesei and Schizophyllum commune. Based on quantitative antifungal activity assays, Chi19MKΔNTerm inhibits the growth of Trichoderma viride with an IC₅₀ value of 0.81 μM.

几丁质同化革兰氏阴性细菌，Lysobacter sp.。MK9-1是从土壤中分离出来的，是糖苷水解酶家族19型几丁质酶（Chi19MK）基因的来源，该基因长933bp，编码311个残基的蛋白质。Chi19MK的推导的氨基酸序列包括信号肽，未表征的序列，碳水化合物结合模块家族的12型几丁质结合域和催化域。Chi19MK的催化域是类似于从那些奇夫的大约60％唐菖蒲伯克霍尔德菌CHB101，几丁质酶N（下巴）从Chitiniphilus shinanonensis SAY3 ^Ť，CHIF从天蓝色链霉菌A3（2），Chi30从链霉菌olivaceoviridisis，嘉从蓝链霉菌SP-27和来自灰链霉菌HUT6037的ChiC。缺乏信号和未表征序列的Chi19MK（Chi19MKΔNTerm）在大肠杆菌Rosetta-gami B（DE3）中表达，导致可溶性级分中具有显着的几丁质酶活性。纯化的Chi19MKΔNTerm水解胶体甲壳质并释放出二糖。此外，Chi19MKΔNTerm抑制里氏木霉和Schizophyllum社的菌丝延伸。根据定量抗真菌活性测定，Chi19MKΔNTerm以0.81μM的IC ₅₀值抑制木霉菌的生长。