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Immunogenicity study of OmpU subunit vaccine against Vibrio mimicus in yellow catfish, Pelteobagrus fulvidraco
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-12-04 , DOI: 10.1016/j.fsi.2020.11.030
Yu Fu , Yong-An Zhang , Jinyu Shen , Jiagang Tu

The outer membrane protein U (OmpU) is a conserved outer membrane protein in a variety of pathogenic Vibrio species and has been considered as a vital protective antigen for vaccine development. Vibrio mimicus (V. mimicus) is the pathogen causing ascites disease in aquatic animals. In this study, the prokaryotically expressed and purified His-tagged OmpU of V. mimicus (His-OmpU) was used as a subunit vaccine. The formalin inactivated V. mimicus, purified His tag (His-tag), and PBS were used as controls. The vaccinated yellow catfish were challenged with V. mimicus at 28 days post-vaccination, and the results showed that the His-OmpU and inactivated V. mimicus groups exhibited much higher survival rates than the His-tag and PBS groups. To fully understand the underlying mechanism, we detected the expression levels of several immune-related genes in the spleen of fish at 28 days post-vaccination and 24 h post-challenge. The results showed that most of the detected immune-related genes were significantly upregulated in His-OmpU and inactivated V. mimicus groups. In addition, we performed the serum bactericidal activity assay, and the results showed that the serum from His-OmpU and inactivated V. mimicus groups exhibited much stronger bactericidal activity against V. mimicus than those of His-tag and PBS groups. Finally, the serum agglutination antibody was detected, and the antibody could be detected in His-OmpU and inactivated V. mimicus groups with the antibody titers increasing along with the time post-vaccination, but not in His-tag or PBS group. Our data reveal that the recombinant OmpU elicits potent protective immune response and is an effective vaccine candidate against V. mimicus in yellow catfish.



中文翻译:

OmpU亚单位疫苗的免疫原性对研究拟态弧菌在黄色的鲶鱼,黄颡鱼

外膜蛋白U(OmpU)是多种致病性弧菌物种中的一种保守的外膜蛋白,被认为是疫苗开发的重要保护性抗原。模拟弧菌V. mimicus)是引起水生动物腹水病的病原体。在这项研究中,原核表达和纯化他标记的OmpU拟态弧菌(HIS-OmpU)作为亚单位疫苗。将福尔马林灭活的拟南芥,纯化的His标签(His-tag)和PBS用作对照。接种疫苗的黄颡鱼用质疑拟态弧菌在28天后接种,结果表明,他的-OmpU和灭活拟态弧菌与His-tag和PBS组相比,这些组的生存率要高得多。为了充分了解其潜在机制,我们在疫苗接种后28天和攻击后24小时检测了鱼脾中几种免疫相关基因的表达水平。结果表明,大多数检测免疫相关基因在他的-OmpU和灭活均显著上调拟态弧菌组。此外,我们进行的血清杀菌活性测定,结果表明,从他的-OmpU和灭活血清拟态弧菌群体表现出更强的杀菌活性对拟态弧菌高于His-tag和PBS组。最后,检测到血清凝集抗体,且该抗体可在他的-OmpU和灭活进行检测拟态弧菌基团与抗体滴度在His-标签或PBS组与时间接种后沿增加,但不。我们的数据表明:重组OmpU引起有效的保护性免疫反应,反对的有效候选疫苗拟态弧菌在黄颡鱼。

更新日期:2020-12-08
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