Growth inhibition by chitosans (with molecular weight 6, 12, 18, 25 and 45 kDa) was demonstrated for 10 filamentous fungi belonging to the families Aspergillaceae, Cladosporiacea, Pleosporaceae, Cordycipitaceae and Microascaceae which are responsible for biodeterioration of tempera paintings and are the dominant members of microbiome from Paintings of Ancient Rus Halls, State Tretyakov Gallery, Russia. The greatest effect was achieved at 2% chitosan in agar medium; its antifungal activity increased in the row: 6‒12‒18‒25 kDa. Chitosans with a molecular weights of 25 and 45 kDa inhibited fungal growth to the same degree. Representatives of Cladosporiaceae and Sordariomycetes (Cordycipitaceae and Microascaceae) showed the greatest sensitivity to chitosan. Some Aspergillaceae were found to exhibit elevated resistance. Investigation of antifungal activity of chitosans incorporated into tempera materials to assess their possible application as art objects antiseptics will be the goal of our further research.

中文翻译：

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寻找有效的壳聚糖类杀菌剂来保护特列季亚科夫国家美术馆展品中的 15 至 16 世纪蛋彩画

壳聚糖（分子量为 6、12、18、25 和 45 kDa）对 10 种丝状真菌的生长抑制作用得到证实，这些真菌属于曲霉科、枝孢菌科、多孢菌科、虫草科和微囊菌科，这些真菌是导致蛋彩画生物退化的主要因素。来自俄罗斯国家特列季亚科夫画廊古代俄罗斯大厅绘画的微生物组成员。在琼脂培养基中加入 2% 壳聚糖时效果最好；其抗真菌活性在行中增加：6-12-18-25 kDa。分子量为 25 和 45 kDa 的壳聚糖对真菌生长的抑制程度相同。Cladosporiaceae 和 Sordariomycetes (Cordycipitaceae 和 Microascaceae) 的代表对壳聚糖表现出最大的敏感性。发现一些曲霉科表现出较高的抗性。