Stimulus-induced in situ forming hydrogels possess the characteristics of easy management and minimal invasiveness via simple injection at target sites with a liquid and easy forming bulk gels. In the present study, a photoreactive monomer, N’-(2-nitrobenzyl)-N-acryloyl glycinamide (NBNAGA) was introduced to modify polyacrylamide (PAM) hydrogel preparation with stimuli responsiveness. Firstly, poly(acrylamide-co-N’-(2-nitrobenzyl)-N-acryloyl glycinamide), P(AM-co-NBNAGA), copolymer solution was prepared via reversible addition fragmentation chain transfer (RAFT) polymerization using the monomers mixture of AM, NBNAGA, and N,N’- methylene bis-acrylamide (BIS). The obtained polymer solution with viscous, transparent, and flowable appearance contained weak single hydrogen bonding and slight chemical crosslinking in a microscopic perspective. Secondly, without further purification, after irradiation using UV light at 365 nm, poly(acrylamide-co-N-acryloyl glycinamide) (P(AM-co-NAGA)) hydrogel conveniently in situ formed due to the cleavage of o-nitrobenzyl groups and the corresponding emergency of dual hydrogen bonding among “uncaged” dual amide moieties. P(AM-co-NAGA) hydrogel depicted both favorable temperature sensitivity and self-healing properties, then the heating induced in vitro release profiles of doxorubicin (DOX) was analyzed.

刺激诱导的原位形成水凝胶具有易于管理和侵入性最小的特点，方法是在目标部位简单注入液体并容易形成块状凝胶。在本研究中，引入了一种光反应性单体N '-（2-硝基苄基）-N-丙烯酰基甘氨酰胺（NB NAGA），以具有刺激响应性的方式改性聚丙烯酰胺（PAM）水凝胶制剂。首先，通过单体的可逆加成断裂链转移（RAFT）聚合反应制备了聚（丙烯酰胺-co- N '-（2-硝基苄基）-N-丙烯酰基甘氨酰胺）共聚物P（AM- co - NB NAGA）共聚物溶液。 AM，NB的混合物NAGA和N，N'-亚甲基双丙烯酰胺（BIS）。所获得的具有粘性，透明和可流动外观的聚合物溶液在微观上包含弱的单个氢键和轻微的化学交联。其次，不经进一步纯化，使用UV光在365nm，聚照射后（丙烯酰胺-共- ñ -丙烯酰基甘氨酰胺）（P（AM- CO -NAGA））水凝胶方便地在原位形成，由于裂解Ò硝基苄基以及“未封端”的双酰胺部分之​​间发生双氢键的相应紧急情况。P（AM- co-NAGA）水凝胶描绘了良好的温度敏感性和自愈特性，然后分析了加热诱导的阿霉素（DOX）的体外释放曲线。