Traditional Chinese medicine (TCM), such as Huanglian-Jie-Du-Tang, a heat-clearing and detoxifying decoction is beneficial to alleviation of inflammation-related diseases. The objective of this study is to uncover the effect and mechanism of heat-clearing, detoxifying and blood stasis removing decoction (HDBD) on the treatment of acute soft tissue injury (STI) which is characterized with excessive inflammatory cascade at the onset. Male Sprague-Dawley (SD) rats with hammer beating served as the in vivo models of acute STI. Haematoxylin-eosin (HE) staining was used for histopathology assessment. The levels of inflammatory factors, including prostaglandin E2 (PGE2), tumor necrosis factor-αumTNF-α), interleukin (IL)-1t and IL-6 were measured by enzyme linked immunosorbent assay (ELISA). Human dermal microvascular endothelium cell line HMEC-1 and rat vascular endothelium cell line RAOEC were used to explore the mechanism in vitro. Luciferase gene reporter assay was applied to determine the relationship between miR-26b-5p and COX2. The results showed that HDBD intervention significantly reduced the temperature difference between the healthy side and affected side of rats with hammer beating, together with the decreased levels of COX2, PGE2, TNF-α, IL-6 and IL-1β, and the increased level of miR-26b-5p. In mechanism, miR-26b-5p targeted COX2 and decreased its expression, leading to significant decreases in the levels of PGE2, TNF-α and IL-6 in RAOEC and HMEC-1 cells. In addition, miR-26b-5p inhibition impaired the effects of HDBD on the suppression of PGE2, TNF-α, IL-6 and IL-1β in vitro. In conclusion, this study revealed that HDBD relieved acute STI via modulating miR-26b-5p/COX2 axis to inhibit inflammation.

中文翻译：

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清热解毒祛瘀汤通过调节MiR-26b-5p / COX2轴抑制炎症减轻急性软组织损伤。

清热解毒汤黄连解毒汤等传统中药对缓解炎症相关疾病十分有益。本研究的目的是揭示清热解毒活血化瘀汤对急性软组织损伤（STI）的治疗作用，该损伤的起因是过度的炎症级联反应。具有锤打的雄性Sprague-Dawley（SD）大鼠用作急性性STI的体内模型。苏木精-曙红（HE）染色用于组织病理学评估。用酶联免疫吸附法（ELISA）检测前列腺素E2（PGE2），肿瘤坏死因子-αumTNF-α，白介素（IL）-1t和IL-6等炎症因子的水平。使用人真皮微血管内皮细胞系HMEC-1和大鼠血管内皮细胞系RAOEC来探索其体外机制。应用萤光素酶基因报告基因测定法确定miR-26b-5p与COX2之间的关系。结果表明，HDBD干预可显着减少锤打大鼠健康侧和患侧之间的温差，同时降低COX2，PGE2，TNF-α，IL-6和IL-1β的水平，并提高水平miR-26b-5p。在机制上，miR-26b-5p靶向COX2并降低其表达，从而导致RAOEC和HMEC-1细胞中PGE2，TNF-α和IL-6的水平明显降低。另外，miR-26b-5p的抑制作用削弱了HDBD在体外对PGE2，TNF-α，IL-6和IL-1β的抑制作用。结论，