A novel graphene oxide (GO)-based carrier was fabricated for the controlled release of Benazepril (BENA). Freeze dried samples of GO-BENA carrier were prepared for controlled drug release at different pHs (pH = 2, 7, and 10) and release kinetics indicate BENA desorption from GO is by Fickian diffusion. The BENA yield from the carrier amounted to ~55% of the adsorbed material in a strongly acidic medium after 50 h. Binding fractions of BENA to 10 mg/L GO was determined for different solution concentrations of the drug. In vitro assays of cell proliferation (WST-1 kit), cell structural integrity (LDH kit) and flow cytometric indicators of necrosis in three different cell lines (CACO-2, SGC-7901, and primary mouse hepatic fibroblast) all demonstrated that the GO carrier had a good biocompatibility. The pH-dependent release sensitivity of the GO-based carrier suggests that it is a potential candidate for use in the controlled release of drugs in the acidic environment of the stomach.

中文翻译：

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pH敏感氧化石墨烯-贝那普利载体的制备作为生物安全控释系统

制备了一种新型氧化石墨烯 (GO) 基载体，用于控制释放贝那普利 (BENA)。制备冷冻干燥的 GO-BENA 载体样品，用于在不同 pH 值（pH = 2、7 和 10）下控制药物释放，释放动力学表明 BENA 从 GO 解吸是通过 Fickian 扩散进行的。50 小时后，在强酸性介质中，来自载体的 BENA 产量达到吸附材料的约 55%。BENA 与 10 mg/L GO 的结合分数是针对不同的药物溶液浓度确定的。三种不同细胞系（CACO-2、SGC-7901 和原代小鼠肝成纤维细胞）中细胞增殖（WST-1 试剂盒）、细胞结构完整性（LDH 试剂盒）和坏死的流式细胞术指标的体外测定均表明GO载体具有良好的生物相容性。