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A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints
Tissue Engineering, Part C: Methods ( IF 3 ) Pub Date : 2020-12-16 , DOI: 10.1089/ten.tec.2020.0161
Adel Tekari 1, 2, 3 , Rainer J Egli 1, 4, 5 , Veit Schmid 6 , Joern Justiz 6 , Reto Luginbuehl 1, 4, 7
Affiliation  

Any significant in vitro evaluation of cartilage tissue engineering and cartilage repair strategies has to be performed under the harsh conditions encountered in vivo within synovial joints. To this end, we have developed a novel automated physiological robot reactor system (PRRS) that is capable of recapitulating complex physiological motions and load patterns within an environment similar to that found in the human knee. The PRRS consists of a mechanical stimulation unit (MSU) and an automatic sample changer (ASC) within an environment control box in which the humidity, temperature, and gas composition are tightly regulated. The MSU has three linear (orthogonal) axes and one rotational degree of freedom (around the z-axis). The ASC provides space for up to 24 samples, which can be allocated to individual stimulation patterns. Cell-seeded scaffolds and ex vivo tissue culture systems were established to demonstrate the applicability of the PRRS to the investigation of the effect of load and environmental conditions on engineering and maintenance of articular cartilage in vitro. The bioreactor is a flexible system that has the potential to be applied for culturing connective tissues other than cartilage, such as bone and intervertebral disc tissue, even though the mechanical and environmental parameters are very different.

中文翻译:

一种能够模拟滑膜关节体内条件的新型生物反应器系统

任何重要的软骨组织工程和软骨修复策略的体外评估都必须在体内遇到的恶劣条件下进行滑膜关节内。为此,我们开发了一种新型自动化生理机器人反应器系统 (PRRS),它能够在类似于人类膝盖的环境中重现复杂的生理运动和负载模式。PRRS 由一个机械刺激单元 (MSU) 和一个自动进样器 (ASC) 组成,位于环境控制箱内,其中的湿度、温度和气体成分受到严格控制。MSU 具有三个线性(正交)轴和一个旋转自由度(围绕 z 轴)。ASC 提供最多可容纳 24 个样本的空间,这些样本可以分配给各个刺激模式。细胞接种支架和离体建立组织培养系统以证明 PRRS 在研究负荷和环境条件对体外关节软骨的工程和维护的影响中的适用性。生物反应器是一个灵活的系统,即使机械和环境参数非常不同,它也有可能用于培养除软骨以外的结缔组织,例如骨和椎间盘组织。
更新日期:2020-12-22
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