Recent biotechnical advances in the in vitro culture of cholangiocytes and generation of bioengineered biliary tissue have a high potential for creating biliary tissue to be used for disease modeling, drug screening, and transplantation. For the past few decades, scientists have searched for a source of cholangiocytes, focused on primary cholangiocytes or cholangiocytes derived from hepatocytes or stem cells. At the same time, the development of scaffolds for biliary tissue engineering for transplantation and modeling of cholangiopathies has been explored. In this review, we provide an overview on the current understanding of cholangiocytes sources, the effect of signaling molecules, and transcription factors on cell differentiation, along with the effects of extracellular matrix molecules and scaffolds on bioengineered biliary tissues, and their application in disease modeling and drug screening.

中文翻译：

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用于疾病建模和治疗的组织工程胆管

体外生物技术的最新进展胆管细胞的培养和生物工程胆管组织的产生具有创建用于疾病建模、药物筛选和移植的胆管组织的巨大潜力。在过去的几十年里，科学家们一直在寻找胆管细胞的来源，重点是原代胆管细胞或源自肝细胞或干细胞的胆管细胞。同时，还探索了胆道组织工程支架的开发，用于胆管病的移植和建模。在这篇综述中，我们概述了目前对胆管细胞来源的理解、信号分子的影响和转录因子对细胞分化的影响，以及细胞外基质分子和支架对生物工程胆管组织的影响，