Abstract Given the strong associations between diet and cancer risk, there is considerable scientific interest in determining whether dietary factors associated with prostate cancer cell implantation may influence epigenetic alternations. The aim of the research was to assess impact of selected trace elements (selenium, zinc and copper) on the kinetics of changes (10-13-14-21 week of life cycle of rats) in the level of 7-methylguanine, 3-methyladenine, 1-methylguanine and 8-oxo-guanine in the urine of rats with implanted prostate cancer cells (LNCaP). Modified nucleobases were determined by validated high performance liquid chromatography coupled to mass spectrometry (LC-MS/MS) method using multiple reaction monitoring (MRM) mode. In the presented model the implantation of rats with cancer cells did not affect the level of the examined biomarkers in the rats’ urine. The level of methyl derivatives was statistically significantly reduced with the age of the examined rats. The implantation of rats with cancer cells results in the appearance of tumors in 71% of the rats obtaining the standard diet and respectively in 25% of those supplemented with selenium. Supplementation with selenium affects both the effectiveness of tumor induction and the concentration of 7-MeG, 3-MeA, 1-MeG and 8-oxoG in urine of the examined rats. These findings show that modified nucleosides can play an important role in cancer prevention.

中文翻译：

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硒、锌和铜对前列腺癌细胞治疗大鼠尿修饰核碱基排泄的影响

摘要 鉴于饮食与癌症风险之间的密切关联，确定与前列腺癌细胞植入相关的饮食因素是否会影响表观遗传改变具有相当大的科学兴趣。该研究的目的是评估选定的微量元素（硒、锌和铜）对 7-甲基鸟嘌呤、3-植入前列腺癌细胞 (LNCaP) 的大鼠尿液中的甲基腺嘌呤、1-甲基鸟嘌呤和 8-氧代鸟嘌呤。修饰的核碱基通过经验证的高效液相色谱与质谱联用 (LC-MS/MS) 方法使用多反应监测 (MRM) 模式进行测定。在所呈现的模型中，将癌细胞植入大鼠并不会影响大鼠尿液中检测到的生物标志物的水平。甲基衍生物的水平随着被检查大鼠的年龄而在统计学上显着降低。将癌细胞植入大鼠后，获得标准饮食的大鼠中有 71% 出现肿瘤，补充硒的大鼠中有 25% 出现肿瘤。补充硒会影响肿瘤诱导的有效性和受检大鼠尿液中 7-MeG、3-MeA、1-MeG 和 8-oxoG 的浓度。这些发现表明，修饰的核苷可以在癌症预防中发挥重要作用。将癌细胞植入大鼠后，获得标准饮食的大鼠中有 71% 出现肿瘤，补充硒的大鼠中有 25% 出现肿瘤。补充硒会影响肿瘤诱导的有效性和受检大鼠尿液中 7-MeG、3-MeA、1-MeG 和 8-oxoG 的浓度。这些发现表明，修饰的核苷可以在癌症预防中发挥重要作用。将癌细胞植入大鼠后，获得标准饮食的大鼠中有 71% 出现肿瘤，补充硒的大鼠中有 25% 出现肿瘤。补充硒会影响肿瘤诱导的有效性和受检大鼠尿液中 7-MeG、3-MeA、1-MeG 和 8-oxoG 的浓度。这些发现表明，修饰的核苷可以在癌症预防中发挥重要作用。