Xenobiotica ( IF 1.8 ) Pub Date : 2020-08-25 , DOI: 10.1080/00498254.2020.1808735 Maki Miyamoto 1 , Yohei Kosugi 1 , Shinji Iwasaki 1 , Ikumi Chisaki 1 , Sayaka Nakagawa 1 , Nobuyuki Amano 1 , Hideki Hirabayashi 1
Abstract
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The unbound fractions in plasma (f up) in two mouse models of humanized liver mice, PXB and humanized TK-NOG mice, were compared with human f up values using equilibrium dialysis method. A good relationship between f up values obtained from PXB mice and humans was observed; the f up of 34/39 compounds (87.2%) in PXB mice were within 3-fold of human f up. In contrast, a weak correlation was observed between human and humanized TK-NOG mouse f up values; the f up of 15/24 compounds (62.5%) in humanized TK-NOG mice were within 3-fold of human f up.
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As different profiles of plasma protein binding (PPB) profiles were observed between PXB and humanized TK-NOG mice, f up evaluation is necessary in each mouse model to utilize these humanized liver mice for pharmacological, drug–drug interaction (DDI), and toxicity studies.
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The unbound fraction in the mixed plasma of human and SCID mouse plasma (85:15) was well correlated with f up in PXB mice (38/39 compounds within a 3-fold). Thus, this artificial PXB mouse plasma could be used to evaluate PPB.
中文翻译:
人源化肝的两种小鼠模型(PXB小鼠和人源化TK-NOG小鼠)中血浆蛋白结合的特征
摘要
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使用平衡透析方法,将两种人源化肝小鼠PXB和人源化TK-NOG小鼠模型中血浆的未结合部分(f up)与人f up值进行比较。观察到从PXB小鼠和人类获得的f up值之间存在良好的关系。所述˚F 向上在PXB小鼠34/39化合物(87.2%)是人类的内3倍˚F 起来。相比之下,人类之间观察到弱的相关性和人源化TK-NOG小鼠˚F 向上值; 所述˚F 向上人的人源化TK-NOG小鼠15/24化合物(62.5%)是在3倍˚F 向上。
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作为血浆蛋白的不同的简档的结合物PXB和人源化TK-NOG小鼠之间观察到(PPB)型材,˚F 了评价是在每个小鼠模型利用药理这些人源化肝脏的小鼠,药物-药物相互作用(DDI),以及毒性必要学习。
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人类和SCID小鼠血浆(85:15)的混合等离子体中未结合的部分以及被与相关˚F 了在PXB小鼠(一内38/39化合物3倍)。因此,这种人工PXB小鼠血浆可用于评估PPB。