Quick and effective DNA extraction from plants for subsequent PCR amplification is sometimes challenging when working across diverse plant taxa that may contain a variety of inhibitory compounds. Time‐consuming methods may be needed to overcome these inhibitory effects as well as the effects of various preservation and collection methods to extract DNA from leaf samples. Our objective was to develop a rapid DNA extraction protocol that could be used with diverse plant taxa to produce high‐quality DNA suitable for downstream PCR applications.

中文翻译：

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使用丙酮从顽固的木本植物类群中快速提取可 PCR 扩增的 DNA

从植物中快速有效地提取 DNA 用于随后的 PCR 扩增有时在处理可能含有多种抑制性化合物的不同植物类群时具有挑战性。可能需要耗时的方法来克服这些抑制作用以及从叶子样本中提取 DNA 的各种保存和收集方法的影响。我们的目标是开发一种快速 DNA 提取方案，该方案可用于多种植物分类群，以产生适用于下游 PCR 应用的高质量 DNA。