Glioblastoma multiforme is the most lethal brain tumor. In the study of mechanisms underlying its development attention has been paid to the microtubular network of its cells, mainly on βIII tubulin, considered as a marker of malignancy. In the present work, we chose to investigate the tubulin code in glioblastoma cells, analyzing the degree of interaction between tubulin post-translational modifications and different proteins associated with them. The pattern of diverse associated proteins such as EB-1, CLIP-170 and kinesin-1 and their degree of co-distribution with the most abundant post-translational tubulin modifications (tyrosination, acetylation and polyglutamylation) were evaluated. Through immunofluorescence we have shown that EB-1, CLIP-170 and kinesin-1 were well detectable in glioblastoma cells. The double fluorescence and colocalization index between the post-translational modifications of tubulin and associated proteins showed that tyrosinated α-tubulin has significantly high affinity with EB-1, CLIP-170 and kinesin-1, while for acetylated and polyglutamylated tubulin, the degree of interaction with the three associated proteins evaluated was less apparent. Data presented in this paper underline the importance of a thorough analysis of the microtubular mechanics in glioblastoma cells. This may suggest new experimental therapeutic approaches able to act more selectively on the microtubular network of cells in this type of cancer.

多形性胶质母细胞瘤是最致命的脑肿瘤。在其发育机制的研究中，人们已经注意到其细胞的微管网络，主要是βIII 微管蛋白，被认为是恶性肿瘤的标志物。在目前的工作中，我们选择研究胶质母细胞瘤细胞中的微管蛋白代码，分析微管蛋白翻译后修饰与与其相关的不同蛋白质之间的相互作用程度。评估了各种相关蛋白（如 EB-1、CLIP-170 和驱动蛋白-1）的模式及其与最丰富的翻译后微管蛋白修饰（酪氨酸化、乙酰化和多聚谷氨酰化）的共分布程度。通过免疫荧光，我们已经表明 EB-1、CLIP-170 和驱动蛋白-1 在胶质母细胞瘤细胞中可以很好地检测到。微管蛋白和相关蛋白的翻译后修饰之间的双荧光和共定位指数表明酪氨酸化的 α-微管蛋白与 EB-1、CLIP-170 和驱动蛋白-1 具有显着的高亲和力，而对于乙酰化和聚谷氨酰化微管蛋白，与评估的三种相关蛋白质的相互作用不太明显。本文中提供的数据强调了对胶质母细胞瘤细胞微管力学进行彻底分析的重要性。这可能表明新的实验性治疗方法能够更有选择性地作用于此类癌症中的细胞微管网络。而对于乙酰化和多聚谷氨酰化微管蛋白，与所评估的三种相关蛋白的相互作用程度不太明显。本文中提供的数据强调了对胶质母细胞瘤细胞微管力学进行彻底分析的重要性。这可能表明新的实验性治疗方法能够更有选择性地作用于此类癌症中的细胞微管网络。而对于乙酰化和多聚谷氨酰化微管蛋白，与所评估的三种相关蛋白的相互作用程度不太明显。本文中提供的数据强调了对胶质母细胞瘤细胞微管力学进行彻底分析的重要性。这可能表明新的实验性治疗方法能够更有选择性地作用于此类癌症中的细胞微管网络。