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Detailed analysis of the fatty acid composition of six plant-pathogenic bacteria
Journal of Chromatography B ( IF 3 ) Pub Date : 2020-12-03 , DOI: 10.1016/j.jchromb.2020.122454
Nina Wiedmaier-Czerny , Dorothee Schroth , Shiri Topman-Rakover , Aya Brill , Saul Burdman , Zvi Hayouka , Walter Vetter

Bacteria show distinct and characteristic fatty acid (FA) patterns which can be modified by environmental conditions. In this study, we cultivated six plant-pathogenic bacteria of agricultural concern and performed a detailed analysis of the fatty acid composition. The study covered four strains of the gram-negative Xanthomonas campestris pathovar (pv) campestris (Xcc), Xanthomonas perforans (Xp), Acidovorax citrulli (Ac) and Pseudomonas syringae pv. tomato (Pst), and two strains of the gram-positive Clavibacter michiganensis subsp. michiganensis (Cmm) and Streptomyces scabies (Ssc). After cultivation, freeze-dried bacteria samples were transesterified and analysed by gas chromatography with mass spectrometry in full scan and selected ion monitoring (SIM) modes. Altogether, 44 different FAs were detected in the six strains with individual contributions of 0.01–43.8% to the total FAs. The variety in the six strains ranged between 12 and 31 individual FAs. The FA composition of Xcc, Xp, Cmm and Ssc were dominated by iso- and anteiso-fatty acids (especially i15:0, a15:0, i16:0), which is typical for most bacteria. In contrast to this, Ac and Pst showed only saturated and monounsaturated FAs. Four of the six bacteria showed similar FA patterns as reported before in the literature. Differences were observed in the case of Cmm where many more FAs were detected in the present study. In addition, to the best of our knowledge, the FA pattern of Xp was presented for the first time.



中文翻译:

六种植物致病菌脂肪酸组成的详细分析

细菌显示出独特且特征性的脂肪酸(FA)模式,可以通过环境条件对其进行修饰。在这项研究中,我们培育了6种农业关注的植物致病细菌,并对脂肪酸组成进行了详细分析。这项研究涵盖四种菌株革兰氏阴性的野油菜黄单pathovar(PV)油菜XCC),黄单胞菌属穿通XP中),食酸菌属citrulliAC)和丁香假单胞菌PV。番茄Pst)和两株革兰氏阳性密歇根杆菌亚种。密执安Cmm的)和链霉菌属疥疮SSC)。培养后,将冻干的细菌样品进行酯交换,并通过气相色谱-质谱联用全扫描和选定离子监控(SIM)模式进行分析。总共在这6个菌株中检测到44种不同的FA,其对总FA的贡献为0.01–43.8%。六个菌株的变异范围在12到31个独立FA之间。的FA组成XCCXP中Cmm的SSC是由主导-和反异脂肪酸(特别是15:0,一个15:0,i 16:0),这是大多数细菌的典型特征。与此相反,AcPst仅显示饱和和单不饱和FA。六个细菌中有四个显示出与文献先前报道的相似的FA模式。在Cmm的情况下观察到差异,在本研究中检测到更多的FA。此外,据我们所知,Xp的FA模式是首次出现。

更新日期:2020-12-26
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