Experimental Gerontology ( IF 3.9 ) Pub Date : 2020-12-03 , DOI: 10.1016/j.exger.2020.111187 Qingling Liu , Chengbin Lei
Background
Alzheimer's disease (AD) is a common neurodegenerative disease with an increasing incidence rate. Numerous microRNAs (miRNAs) have been found to be involved in AD progression. This study aimed to investigate the expression and diagnostic value of microRNA-331-3p (miR-331-3p) in AD patients and to explore the effects of miR-331-3p on neuronal viability and neuroinflammation.
Methods
This study recruited AD patients and used Aβ1–40 treated SH-SY5Y cells mimicking AD characteristics. The expression of miR-331-3p was estimated using reverse transcription quantitative PCR. A receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic value of miR-331-3p, and the correlation of miR-331-3p with patients' Mini-Mental State Examination (MMSE) scores and serum proinflammatory cytokines were analyzed. The effects of miR-331-3p on neuronal viability and inflammatory response were explored in SH-SY5Y cells by in vitro analysis.
Results
In AD patients and Aβ1–40 treated SH-SY5Y cells, the expression of miR-331-3p was significantly downregulated. Serum miR-331-3p had certain diagnostic potential and was correlated with the MMSE scores and serum proinflammatory cytokine levels of AD patients. In Aβ1–40-treated SH-SY5Y cells, the overexpression of miR-331-3p enhanced cell viability and inhibited inflammatory responses.
Conclusion
The data of this study indicated that serum expression of miR-331-3p is decreased in AD patients, and is correlated with the MMSE scores and proinflammatory cytokine levels of AD patients. In addition, miR-331-3p can regulate the cell viability and the expression of pro-inflammatory cytokines of Aβ1–40 treated SH-SY5Y cells, indicating the potential neuroprotective role of miR-331-3p.
中文翻译:
miR-331-3p通过改善细胞活力和炎性标志物表达的神经保护作用:血清miR-331-3p水平与阿尔茨海默氏病的诊断和严重程度的相关性
背景
阿尔茨海默氏病(AD)是一种常见的神经退行性疾病,发病率不断上升。已经发现许多microRNA(miRNA)参与AD进程。本研究旨在探讨microRNA-331-3p(miR-331-3p)在AD患者中的表达和诊断价值,并探讨miR-331-3p对神经元生存能力和神经炎症的影响。
方法
本研究招募了AD患者和用于Aβ 1 - 40处理的SH-SY5Y细胞模仿AD的特性。使用逆转录定量PCR评估miR-331-3p的表达。使用接收器操作特征(ROC)分析来评估miR-331-3p的诊断价值,并分析miR-331-3p与患者的小精神状态检查(MMSE)得分和血清促炎细胞因子的相关性。通过体外分析探讨了miR-331-3p对SH-SY5Y细胞神经元活力和炎症反应的影响。
结果
在AD患者和Aβ 1 - 40处理的SH-SY5Y细胞,的miR-331-3p的表达显著下调。血清miR-331-3p具有一定的诊断潜能,并与AD患者的MMSE评分和血清促炎细胞因子水平相关。在Aβ 1 - 40 -处理的SH-SY5Y细胞,的miR-331-3p增强细胞生存力和抑制炎症反应的过表达。
结论
这项研究的数据表明,miR-331-3p的血清表达在AD患者中降低,并且与AD患者的MMSE评分和促炎细胞因子水平相关。此外,的miR-331-3p能调节细胞活力和Aβ的促炎细胞因子的表达1 - 40处理的SH-SY5Y细胞,这表明的miR-331-3p的潜在的神经保护作用。