For a better understanding of the effect of drugs and their interaction with cells and tissues, there is a need for in vitro and ex vivo model systems which enables studying these events. There are several in vitro methods available to evaluate the antioxidant activity; however, these methods do not factor in the complex in vivo physiology. Here we present an intestinal tissue modified oxygen electrode, used for the detection of the antioxidant effect of orally administered drugs in the presence of H₂O₂. Antioxidants are essential in the defense against oxidative stress, more specifically against reactive oxygen species such as H₂O_2. Due to the presence of native catalase in the intestine, with the tissue-based biosensor we were able to detect H₂O₂ in the range between 50 and 500 µM. The reproducibility of the sensor based on the calculated relative standard deviations was 15 ± 6%. We found that the O₂ production by catalase from H₂O₂ was reduced in the presence of a well-known antioxidant, quinol. This indirectly detected antioxidant activity was also observed in the case of orally administered drugs with a reported anti-inflammatory effect such as mesalazine and paracetamol, while no antioxidant activity was recorded with aspirin and metformin.

为了更好地理解药物的作用及其与细胞和组织的相互作用，需要能够研究这些事件的体外和离体模型系统。有几种体外方法可用于评估抗氧化活性。然而，这些方法没有考虑复杂的体内生理。在这里，我们介绍了一种肠组织修饰的氧电极，用于检测H ₂ O ₂存在下口服药物的抗氧化作用。抗氧化剂对于抵抗氧化应激（尤其是抵抗活性氧，例如H ₂ O _2）至关重要_。由于肠道中存在天然过氧化氢酶，使用基于组织的生物传感器，我们能够检测到50至500 µM之间的H ₂ O ₂。根据计算出的相对标准偏差，传感器的重现性为15±6％。我们发现，在众所周知的抗氧化剂喹诺醇的存在下，过氧化氢酶从H ₂ O ₂产生的O ₂减少了。在口服已报道的具有消炎作用的药物（如美沙拉嗪和扑热息痛）中也观察到了这种间接检测到的抗氧化活性，而阿司匹林和二甲双胍未记录到抗氧化活性。