Journal of Parasitology ( IF 1.3 ) Pub Date : 2020-12-01 , DOI: 10.1645/19-196 Harun Kaya Kesik 1 , Seyma Gunyakti Kilinc 1 , Figen Celik 2 , Sami Simsek 2 , Haroon Ahmed 3
Echinococcus multilocularis is a parasite species of zoonotic importance which can be fatal to humans and causes Alveolar Echinococcosis (AE). This report describes the development of a cyst from the liver of a wild boar and molecular confirmation of its identification. The cyst material was obtained from the liver of a wild boar killed by hunters. Genomic DNA was extracted from the germinal layer of the cyst material, and 875 bp mitochondrial cytochrome c oxidase subunit I (COI) gene fragment was amplified by PCR and sequenced. A BLAST search matched 100% with published Echinococcus multilocularis sequences. This study confirms the occurrence of E. multilocularis in a wild boar for the first time in Turkey.
中文翻译:
野猪棘球oc虫分子诊断的病例研究及其在土耳其的公共卫生意义
多叶棘球oc虫是一种具有人畜共患意义的寄生虫,可能对人类致命,并引起肺泡棘球cc病(AE)。该报告描述了野猪肝脏囊肿的发展及其鉴定的分子确认。囊肿物质是从猎人杀死的野猪肝脏中获得的。从囊肿材料的生发层提取基因组DNA,并通过PCR扩增875 bp的线粒体细胞色素c氧化酶亚基I(COI)基因片段并进行测序。BLAST搜索将100%与公开的多球棘球oc菌序列匹配。这项研究证实了土耳其野猪中首次出现了多叶大肠杆菌。