Abstract

S-adenosylmethionine (SAM) is an important compound in living organisms and has a number of different roles, such as polyamine synthesis. It can also be used for the treatment of diseases. Gene targeting with homologous recombination in Komagataella phaffii (K. phaffii) created DAS1 and DAS2 gene knockout strains, which use the methanol pathway to generate more ATP and increase SAM production. These strains showed an increase in the flux of methanol oxidation and an increase in ATP production. The gene knockout retarded the cell growth in the late phase of induction, did not influence the total methanol consumption or the activity of SAM synthetase, but did significantly increase the yield of SAM by 43.7%. The metabolic flux of methanol oxidation is increased by DAS1 and DAS2 gene knockout, which leads to an increase in ATP and higher SAM production.

摘要

S-腺苷甲硫氨酸（SAM）是生物体中的重要化合物，具有多种不同的作用，例如多胺合成。它也可以用于治疗疾病。基因靶向与Kophaataella phaffii（K. phaffii）中的同源重组产生DAS1和DAS2。基因敲除菌株，利用甲醇途径产生更多的ATP并增加SAM的产量。这些菌株显示出甲醇氧化通量的增加和ATP产量的增加。该基因敲除在诱导后期延迟了细胞生长，不影响总甲醇消耗或SAM合成酶的活性，但确实使SAM的产率显着增加了43.7％。DAS1和DAS2基因敲除会增加甲醇氧化的代谢通量，从而导致ATP的增加和更高的SAM产量。