Abstract

Several components of mushroom compost (wheat straw, chicken manure) can be contaminated with mycotoxins posing food health risks to mushroom consumers. To assess the relevance of such contaminations high-throughput analytical methods are needed. In this study, two sample preparation approaches, dilute & shoot (D&S) and modified citrate buffered Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS) were compared in terms of extraction efficiency and matrix effect in case of 13 mycotoxins in complex matrices–wheat straw, the growing media and button mushrooms (Agaricus bisporus)–of mushroom cultivation using high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). D&S method resulted in recoveries of LB medium, button mushroom and compost for ≥60% in case of all investigated mycotoxins except for DON-3G. However, using modified citrate buffered QuEChERS with 2% acidification of the extraction solvent showed the complete loss of strongly polar DON-3G and fumonisin B₁ (FB₁). The investigated matrices had suppressive effect on ionization in all target mycotoxins except for FB₁. Regarding the use of isotopologues to compensate matrix effect, even U-[¹³C₁₅]-DON and U-[¹³C₂₄]-T-2 can also be used to quantify their related metabolites in the studied matrices, using internal standard method.

摘要

蘑菇堆肥的几种成分（麦秸，鸡粪）可能会被霉菌毒素污染，对蘑菇消费者构成食品健康风险。为了评估此类污染物的相关性，需要高通量的分析方法。在这项研究中，比较了13种霉菌毒素在复杂条件下的提取效率和基质效应，比较了两种样品制备方法，即稀释和射击（D＆S）以及改性柠檬酸盐快速，简便，廉价，有效，坚固，安全的缓冲液（QuEChERS）。基质–小麦秸秆，生长培养基和蘑菇（Agaricus bisporus）–高效液相色谱串联质谱（HPLC-MS / MS）进行蘑菇栽培 D＆S方法对所有调查的霉菌毒素（DON-3G除外）的回收率均达到60％以上，包括LB培养基，蘑菇和堆肥。但是，使用经柠檬酸缓冲的QuEChERS修饰的提取溶剂2％的酸化溶液，表明完全失去了强极性DON-3G和伏马菌素B ₁（FB ₁）。研究的基质对除FB ₁以外的所有目标真菌毒素均具有抑制电离的作用。关于使用同位素共聚物补偿基质效应，甚至使用U- [ ¹³ C ₁₅ ] -DON和U- [ ¹³ C ₂₄] -T-2还可以使用内标方法定量分析所研究基质中的相关代谢物。