The environmental reservoirs of different serogroups of Vibrio cholerae causing cholera in the flowing freshwater bodies of the tribal areas of Odisha are not known. So the present study was conducted from June 2017 to March 2020 to find out the environmental reservoirs of V. cholerae serogroups in the water and plankton samples collected from the river, nala, stream and chua from Rayagada district. Similarly, rectal swabs were collected from diarrhoea patients and correlation was established among the V. cholerae strains isolated from diarrhoea patients and environmental V. cholerae isolates through routine culture, different multiplex PCR assays and pulse field gel electrophoresis (PFGE) analysis using standard techniques. The multiplex PCR assays on biotypes and different toxic genes exhibited similar correlation between the clinical and water isolates, which was further strengthened by PFGE analysis. The planktonic DNA was positive for ctxA gene which established that the environmental water bodies were the reservoirs for virulence genes of V. cholerae serogroups. The detection of environmental reservoirs of V. cholerae serogroups in temporarily stagnant condition of water; partially encircled by stones, and near the bank of the river, nala and stream were the reservoirs which is a rare report from Odisha, India and Globe.

中文翻译：

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印度东部奥里萨邦部落地区流动淡水环境中霍乱弧菌血清群的环境水库

在奥里萨邦部落地区的流动淡水体中引起霍乱的不同霍乱弧菌血清群的环境库尚不清楚。因此，本研究于 2017 年 6 月至 2020 年 3 月进行，以了解从 Rayagada 地区的河流、nala、溪流和 Chua 采集的水中和浮游生物样本中霍乱弧菌血清群的环境库。同样，从腹泻患者身上采集直肠拭子，并在从腹泻患者身上分离出的霍乱弧菌菌株与环境霍乱弧菌之间建立了相关性通过常规培养、不同的多重 PCR 测定和使用标准技术的脉冲场凝胶电泳 (PFGE) 分析分离分离物。对生物型和不同毒性基因的多重 PCR 检测在临床分离物和水分离物之间表现出相似的相关性，PFGE 分析进一步加强了这一点。浮游生物 DNA 对ctx A 基因呈阳性，这表明环境水体是霍乱弧菌血清群毒力基因的储存库。在水暂时停滞的条件下检测霍乱弧菌血清群的环境库；部分被石头包围，靠近河岸、纳拉和溪流是水库，这是奥里萨邦、印度和环球的罕见报道。