Abstract The study involves 73 female participants including 40 healthy young (19-37 years), 17 non-diabetic elderly (50 to 80 years) and 16 diabetic elderly (54 to 91 years). The main purpose of this study is to evaluate the water content of human dermis by in vivo confocal Raman spectroscopy. The results pointed out that the total water content of the dermis is 74.4% ± 3.7, 71.8% ± 2.4, and 73.7% ± 3.7 for diabetic elderly, healthy young adults, and healthy elderly women, respectively. The difference in total water content between elderly diabetic women vs healthy young women and for healthy elderly women vs healthy young women was 2.7 and 1.6%, respectively. A way to analyze only bounded water (independent of the use of skin – tools) in the amino acids chain of collagen was performed subtracting the Raman water spectrum of the mean Raman spectra of the free groups of participants. The bounded water in the collagen fibers was found at 3515 – 3700 cm-1 and corresponds to 31.84% in healthy young, 34.69% in healthy elderly, and 33.47 % in diabetic elderly women. This study showed that both the total human dermal water concentration and bounded water could be measured in vivo using confocal Raman spectroscopy.

中文翻译：

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用共聚焦拉曼光谱法在体内测定皮肤按时间顺序老化的皮肤含水量

摘要 该研究涉及 73 名女性参与者，包括 40 名健康青年（19-37 岁）、17 名非糖尿病老年人（50 至 80 岁）和 16 名糖尿病老年人（54 至 91 岁）。本研究的主要目的是通过体内共聚焦拉曼光谱评估人体真皮的含水量。结果指出，糖尿病老人、健康青年和健康老年妇女真皮的总含水量分别为74.4%±3.7、71.8%±2.4和73.7%±3.7。老年糖尿病女性与健康年轻女性以及健康老年女性与健康年轻女性的总水分含量差异分别为 2.7% 和 1.6%。一种仅分析胶原蛋白氨基酸链中结合水（独立于皮肤工具的使用）的方法减去自由参与者组的平均拉曼光谱的拉曼水光谱。胶原纤维中的结合水位于 3515 – 3700 cm-1，对应于健康年轻人的 31.84%、健康老年人的 34.69% 和糖尿病老年女性的 33.47%。该研究表明，使用共焦拉曼光谱可以在体内测量总人真皮水浓度和结合水。