Golgi apparatus plays an important role in the final processing and packaging of cell secretions. Herein, we first present a new strategy using 2′,3′-O-isopropylideneadenosine (Ade) acting as active site of many molecules in the Golgi and tetraphenylethylene (TPE) with aggregation-induced emission (AIE) characteristics to design and synthesize a fluorescence probe termed as TPE-Ade. The fluorescence intensity of TPE-Ade in aqueous solution is enhanced by 160 times and thus AIE effect has been obviously confirmed. Furthermore, the theoretical calculation results demonstrate that aggregation enhanced fluorescence of TPE-Ade could be attributed to the suppression of non-radiative decay channel due to the intramolecular rotation hinder. Subsequently, TPE-Ade performs excellent in co-localization imaging experiment in targeting Golgi apparatus, with the Pearson’s co-localization coefficient of 0.86. Moreover, TPE-Ade shows better photostability in contrast to Golgi-Tracker Red. This work may develop into a fluorescence imaging tool based on a new Golgi apparatus localization group.

高尔基体在细胞分泌物的最终加工和包装中起着重要作用。在这里，我们首先提出一种新的策略，使用2'，3'-O-异丙基亚氨腺苷（Ade）作为高尔基体中许多分子的活性位点，并利用具有聚集诱导发射（AIE）特征的四苯基乙烯（TPE）设计和合成荧光探针称为TPE-Ade。水溶液中TPE-Ade的荧光强度提高了160倍，因此可以明显确认AIE效应。此外，理论计算结果表明，由于分子内旋转阻碍，TPE-Ade的聚集增强的荧光可以归因于对非辐射衰变通道的抑制。随后，TPE-Ade在针对高尔基体的共定位成像实验中表现出色，皮尔森的共定位系数为0.86。此外，与高尔基-Tracker Red相比，TPE-Ade具有更好的光稳定性。这项工作可能会发展成为基于新的高尔基体定位小组的荧光成像工具。