The ability of infectious laryngotracheitis virus (ILTV) to replicate in organs outside of the upper respiratory tract and conjunctiva associated-lymphoid tissues is still not well understood. This study investigated the tissue distribution of an Australian field strain of ILTV (class 9) on birds experimentally inoculated via eye-drop at 7 days of age by using quantitative PCR (qPCR) and immunohistochemistry. Tissues including conjunctiva, caecal tonsil, kidney, liver, lung, spleen, thymus, trachea and blood were collected from sham-inoculated (control group; n = 2) and ILTV-inoculated (n = 8) birds at 7 days post-inoculation (dpi). Blood was collected from 13 infected birds at 14 dpi and fractionated using ficoll-paque. At 7 dpi, the highest detection rate and genomic copies (GC) were in conjunctiva (8/8; 8.08 ± 0.48 log₁₀ GC/mg) followed by trachea (8/8; 4.64 ± 0.48) and thymus (8/8; 4.52 ± 0.48), kidney (8/8; 3.97 ± 0.48), lung (8/8; 3.65 ± 0.48), spleen (8/8; 3.55 ± 0.48), liver (8/8; 3.51 ± 0.48), caecal tonsil (7/8; 3.76 ± 0.48) and plasma (4/8; 2.40 ± 0.48 log₁₀ GC/ml). ILTV antigen was only detected in conjunctiva (7/8), trachea (6/8) and lung (4/8) samples. At 14 dpi, ILTV detection rate and genomic copies in buffy coat cells were 12/13 and 2.86 ± 0.39 log₁₀ GC/mg, respectively while those of plasma were 11/13 and 4.29 ± 0.39 log₁₀ GC/ml and red blood cell were 3/13 and 0.36 ± 0.39 log₁₀ GC/mg. In conclusion, ILTV DNA was detected in a wide range of tissues and blood fractions but ILTV antigen was only detected in respiratory organs and conjunctiva.

感染性喉气管炎病毒（ILTV）在上呼吸道和结膜相关淋巴组织之外的器官中复制的能力仍未得到很好的了解。这项研究使用定量PCR（qPCR）和免疫组织化学方法，对7天龄通过滴眼液进行实验性接种的澳大利亚ILTV（9类）澳大利亚野外菌株在鸟类上的组织分布进行了研究。从假接种（对照组；n  = 2）和ILTV接种（n）中收集结膜，盲肠扁桃体，肾脏，肝，肺，脾，胸腺，气管和血液等组织。 = 8）接种后7天（dpi）的家禽。以14 dpi从13只受感染的鸟类收集血液，并使用ficoll-paque分馏。在7 dpi时，结膜（8/8; 8.08±0.48 log ₁₀ GC / mg）的检出率和基因组拷贝数（GC）最高，其次是气管（8/8; 4.64±0.48）和胸腺（8/8; 10）。 4.52±0.48），肾（8/8; 3.97±0.48），肺（8/8; 3.65±0.48），脾（8/8; 3.55±0.48），肝（8/8; 3.51±0.48），盲肠扁桃体（7/8; 3.76±0.48）和血浆（4/8; 2.40±0.48 log ₁₀ GC / ml）。ILTV抗原仅在结膜（7/8），气管（6/8）和肺（4/8）样本中检测到。在14 dpi时，血沉棕黄层细胞中ILTV的检出率和基因组拷贝分别为₁₀ GC / mg的12/13和2.86±0.39 log ，而血浆的ILTV检出率为11/13和4.29±0.39 log₁₀ GC / ml和红细胞分别为3/13和0.36±0.39 log ₁₀ GC / mg。总之，在广泛的组织和血液中检测到ILTV DNA，但仅在呼吸器官和结膜中检测到ILTV抗原。