The in vitro antagonist growth effect of bifidobacteria were evaluated on periodontal bacteria. Bifidobacterium longum, Bifidobacterium lactis and Bifidobacterium infantis biofilms were grown in single, double or triple combinations with putative periodontal pathogens P. gingivalis and F. nucleatum or beneficial bacteria S. oralis for 24, 72 and 168 h and the total counts were analyzed by checkerboard DNA-DNA hybridization. The results showed that B. infantis and B. lactis, as single species, demonstrated the best antagonist effect on F. nucleatum and P. gingivalis and no influence on S. oralis growth at 168 h. All the double combinations of bifidobacteria tested demonstrated an inhibitory effect on F. nucleatum (72 h) and P. gingivalis (168 h) and did not affect S. oralis counts at any time. In conclusion, B. lactis and B. infantis alone or in double combinations have antagonist effect on periodontopathogens biofilms, at different time points, and minimal influence on S. oralis growth.

在体外对牙周病菌双歧杆菌的生长拮抗剂效果进行了评价。将长双歧杆菌，乳酸双歧杆菌和婴儿双歧杆菌生物膜与推定的牙周病原体牙龈卟啉单胞菌和核镰刀菌或有益的口腔链球菌S，O，B分别以单，双或三联培养24、72和168 h，并通过棋盘格分析总计数DNA-DNA杂交。结果表明，婴儿双歧杆菌和乳酸双歧杆菌作为单一物种，对核镰刀菌和牙龈卟啉单胞菌表现出最佳的拮抗作用。168 h对口链霉菌的生长无影响。所有双歧杆菌的双组合进行试验表现出对具有抑制作用具核（72小时）和牙龈卟啉（168 h）和不影响口腔链球菌随时计数。总之，单独的乳双歧杆菌和婴儿双歧杆菌在不同时间点对牙周病原生物膜具有拮抗作用，并且对口腔链球菌的生长影响最小。