To reduce dependence on petroleum, the biosynthesis of important chemicals from simple substrates using industrial microorganisms has attracted increased attention. Metabolic engineering of Saccharomyces cerevisiae offers a sustainable and flexible alternative for the production of various chemicals. As a key metabolic intermediate, malonyl-CoA is a precursor for many useful compounds. However, the productivity of malonyl-CoA derivatives is restricted by the low cellular level of malonyl-CoA and enzymatic performance. In this review, we focused on how to increase the intracellular malonyl-CoA level and summarize the recent advances in different metabolic engineering strategies for directing intracellular malonyl-CoA to the desired malonyl-CoA derivatives, including strengthening the malonyl-CoA supply, reducing malonyl-CoA consumption, and precisely controlling the intracellular malonyl-CoA level. These strategies provided new insights for further improving the synthesis of malonyl-CoA derivatives in microorganisms.

为了减少对石油的依赖，使用工业微生物从简单的底物生物合成重要化学品引起了越来越多的关注。酿酒酵母的代谢工程为各种化学品的生产提供了可持续和灵活的替代方案。作为一种关键的代谢中间体，丙二酰辅酶 A 是许多有用化合物的前体。然而，丙二酰辅酶A衍生物的生产力受到丙二酰辅酶A的低细胞水平和酶促性能的限制。在这篇综述中，我们重点讨论了如何提高细胞内丙二酰辅酶 A 的水平，并总结了将细胞内丙二酰辅酶 A 导向所需的丙二酰辅酶 A 衍生物的不同代谢工程策略的最新进展，包括加强丙二酰辅酶 A 的供应，减少丙二酰辅酶 A 的供应。 -CoA消耗，精确控制细胞内丙二酰辅酶A水平。这些策略为进一步改善微生物中丙二酰辅酶A衍生物的合成提供了新的见解。