Abstract

Fluctuations in physiological processes are a key property of biosystems; these fluctuations can play both positive and negative roles in living organisms. In particular, fluctuations in the activity of ion channels can increase sensitivity of the receptors and modulate the activity of neural networks. At the same time, a possible influence of fluctuations on the electrical activity in plants has not been investigated as yet. The aim of this work was a theoretical analysis of the influence of fluctuations in the plasma membrane H⁺‑ATPase activity on the low-temperature-induced electrical responses in a plant cell. The mathematical model of plant electrogenesis developed by the authors earlier, which was modified by description of fluctuations in the H⁺-ATPase activity, was used in this study. Fluctuations in the H⁺-ATPase activity were described by multiplying the H⁺ flux through the H⁺-ATPase by an additional random value with a normal distribution (its specific values were recalculated every 1 s; the mean value was 1). The analysis of the model has shown that an increase in the magnitude of fluctuations in the H⁺-ATPase activity was accompanied by (i) a non-linear increase of fluctuations in the membrane potential at rest; (ii) a decrease in the threshold of generation of simulated action potential when analyzing individual cells simulated by the model; and (iii) modification of low-temperature-induced changes in the membrane potential when averaging electrical activity over a large sample of cells. The obtained results theoretically predict that fluctuations in the H⁺-ATPase activity can decrease the threshold of generation of electrical responses in a plant cell upon cooling.

中文翻译：

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波动对血浆细胞膜H + -ATPase活性影响的低温诱导植物细胞电响应的理论分析

摘要

生理过程的波动是生物系统的关键特性。这些波动在活生物体中既可以发挥积极作用，也可以发挥消极作用。特别地，离子通道活性的波动可增加受体的敏感性并调节神经网络的活性。同时，尚未研究波动对植物电活动的可能影响。这项工作的目的是对质膜H ⁺ -ATPase活性波动对植物细胞中低温诱导的电响应的影响进行理论分析。作者先前开发的植物电发生的数学模型，通过描述H ⁺的波动进行了修改。-ATPase活性被用于这项研究。在H波动⁺ -ATPase活性由H乘以描述⁺通量通过H ⁺通过与正态分布的附加随机值-ATPase（其具体值被重新计算每1秒;平均值为1）。对模型的分析表明，H ⁺的波动幅度增加了。-ATPase活性伴随（i）静止时膜电位波动的非线性增加；（ii）分析模型模拟的单个细胞时，模拟动作电位的产生阈值降低；（iii）当平均大量细胞样本的电活动时，低温诱导的膜电位变化的改变。从理论上获得的结果预测，H ⁺ -ATPase活性的波动可降低冷却后植物细胞中电响应产生的阈值。