Significance: Prenatal exposure to ethanol causes several morphological and neurobehavioral deficits. While there are some studies on the effects of ethanol exposure on blood flow, research focusing on acute changes in the microvasculature is limited. Aim: The first aim of this study was to assess the dose-dependent changes in murine fetal brain microvasculature of developing fetuses in response to maternal alcohol consumption. The second aim was to quantify changes in vasculature occurring concurrently in the mother’s hindlimb and the fetus’s brain after maternal exposure to alcohol. Approach: Correlation mapping optical coherence angiography was used to evaluate the effects of prenatal exposure to different doses of ethanol (3, 1.5, and 0.75 g / kg) on murine fetal brain vasculature in utero. Additionally, simultaneous imaging of maternal peripheral vessels and the fetal brain vasculature was performed to assess changes of the vasculature occurring concurrently in response to ethanol consumption. Results: The fetal brain vessel diameters (VDs) decreased by ∼47 % , 30%, and 14% in response to ethanol doses of 3, 1.5, and 0.75 g / kg, respectively. However, the mother’s hindlimb VD increased by 63% in response to ethanol at a dose of 3 g / kg. Conclusions: Results showed a dose-dependent reduction in vascular blood flow in fetal brain vessels when the mother was exposed to ethanol, whereas vessels in the maternal hindlimb exhibited concurrent vasodilation.

中文翻译：

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产前乙醇暴露引起的小鼠胎儿大脑和母体四肢微血管变化的剂量反应分析

意义：产前接触乙醇会导致多种形态和神经行为缺陷。虽然有一些关于乙醇暴露对血流影响的研究，但专注于微血管系统急性变化的研究是有限的。目的：本研究的第一个目的是评估发育中胎儿的小鼠胎儿脑微血管系统对母体饮酒的反应的剂量依赖性变化。第二个目的是量化母亲接触酒精后母亲后肢和胎儿大脑中同时发生的脉管系统变化。方法：相关映射光学相干血管造影用于评估产前暴露于不同剂量乙醇（3、1.5 和 0.75 g/kg）对子宫内小鼠胎儿脑血管系统的影响。此外，对母体外周血管和胎儿脑脉管系统进行同时成像，以评估因乙醇消耗而同时发生的脉管系统变化。结果：在 3、1.5 和 0.75 g/kg 的乙醇剂量下，胎儿脑血管直径 (VD) 分别减少了 ~47%、30% 和 14%。然而，母亲的后肢 VD 对 3 g/kg 剂量的乙醇的反应增加了 63%。结论：结果显示，当母亲暴露于乙醇时，胎儿脑血管中的血管血流量呈剂量依赖性减少，而母亲后肢的血管同时出现血管舒张。分别响应 3、1.5 和 0.75 g/kg 的乙醇剂量，胎儿脑血管直径 (VD) 减少了 ~47%、30% 和 14%。然而，母亲的后肢 VD 对 3 g/kg 剂量的乙醇的反应增加了 63%。结论：结果显示，当母亲暴露于乙醇时，胎儿脑血管中的血管血流量呈剂量依赖性减少，而母亲后肢的血管同时出现血管舒张。分别响应 3、1.5 和 0.75 g/kg 的乙醇剂量，胎儿脑血管直径 (VD) 减少了 ~47%、30% 和 14%。然而，母亲的后肢 VD 对 3 g/kg 剂量的乙醇的反应增加了 63%。结论：结果显示，当母亲暴露于乙醇时，胎儿脑血管中的血管血流量呈剂量依赖性减少，而母亲后肢的血管同时出现血管舒张。