The extract of G. officinalis was separated by five major fractions and the antitumor activity of hydrophobic fractions has been established. For the most active fractions spectral investigations of the main component were performed using UV-Vis electronic spectra, circular dichroism (CD), Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. On the basis of spectral characteristics, it was demonstrated that the main components of the fractions 4 and 5 of G. officinalis providing its biological activity, highly likely, are of flavonoid nature. Based on the NMR data we propose the structure of the aglycon moiety of the main component from fraction 4 as 4,2',5'-trihydroxychalcone. The fraction 5 is the most active, the antitumor effect of which is implemented through cytotoxic, cytostatic, apoptotic and autophagosomal activity in respect to human kidney cancer tumor cells.

中文翻译：

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含黄酮的Gratiola officinalis L.提取物各部分的分离，光谱表征和生物活性

秦G的提取物被分为五个主要部分，疏水部分的抗肿瘤活性已经确定。对于最活跃的馏分，主要成分的光谱研究是使用UV-Vis电子光谱，圆二色性（CD），傅里叶变换红外光谱（FTIR）和核磁共振（NMR）光谱进行的。根据光谱特征，证明了提供其生物学活性的山茱G组分4和5的主要成分很可能是类黄酮。根据NMR数据，我们提出了馏分4中主要成分的糖苷配基部分的结构为4,2'，5'-三羟基查耳酮。馏分5是最活跃的，其抗肿瘤作用是通过细胞毒性，抑制细胞生长，