Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

中文翻译：

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在水凝胶珠上形成的脂质双层，用于单离子通道记录。

离子通道蛋白在各种细胞功能中起着重要作用，使其成为有吸引力的药物靶标。人工脂质双层记录是一种用于以高灵敏度和准确性测量通道蛋白的离子转运活性的技术。但是，测量效率低。为了提高效率，我们开发了一种方法，该方法允许我们在水凝胶珠上形成双层并迅速记录通道电流。我们通过测量各种类型的通道的活性来测试我们的系统，这些通道包括短杆菌肽，缩水芹素，α-溶血素，电压依赖性阴离子通道1（VDAC1），电压和钙激活的大电导钾通道（BK通道），和青霉链霉菌的钾通道（KcsA频道）。我们确认了增强测量效率和测量系统小型化的能力。