Simple sequence repeats (SSRs) are widely used as markers for the assessment of genetic diversity and marker-assisted breeding. In a previous study, two SSRs (GCA and CCA), were found in the genomic sequence of Larix (La) SCL6, which plays important roles in the growth and development of Larix kaempferi (Lamb.) Carr. In this study, we analyzed the polymorphisms of these two SSRs in the L. kaempferi population. We found that each SSR had five different polymorphisms, among which (GCA)₇ and (CCA)₇ were predominant. In addition, 12 haplotypes were detected, with (GCA)₇(CCA)₇ having the highest frequency. Furthermore, we detected the haplotypes of LaSCL6 in mature trees and their seeds and analyzed the relationships between parents and offspring. The expression patterns of five LaSCL6 alleles were analyzed and they showed balanced expression during vegetative development. Taken together, these findings not only provide more genetic information on LaSCL6, but also provide a candidate marker for genetic studies and breeding.

中文翻译：

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落叶松（Larik kaempferi（Lamb。）Carr。）中LaSCL6等位基因的鉴定和表征。基于简单序列重复和等位基因表达的分析

简单序列重复（SSR）被广泛用作评估遗传多样性和标记辅助育种的标记。在先前的研究中，在Larix（La）SCL6的基因组序列中发现了两个SSR（GCA和CCA），它们在Larix kaempferi（Lamb。Carr）的生长和发育中起着重要作用。在这项研究中，我们分析了L. kaempferi种群中这两个SSR的多态性。我们发现每个SSR具有五个不同的多态性，其中（GCA）₇和（CCA）₇是主要的。此外，检测到12个单倍型，其中（GCA）₇（CCA）₇具有最高的频率。此外，我们检测了成熟树及其种子中LaSCL6的单倍型，并分析了父母与后代之间的关系。分析了五个LaSCL6等位基因的表达模式，它们在营养发育过程中表达均衡。综上，这些发现不仅提供了有关LaSCL6的更多遗传信息，而且还为遗传研究和育种提供了候选标记。