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Alleviation of lead toxicity and phytostimulation in perennial ryegrass by the Pb-resistant fungus Trichoderma asperellum SD-5
Functional Plant Biology ( IF 3 ) Pub Date : 2020-12-01 , DOI: 10.1071/fp20237
Xin Sun , Mingjie Sun , Ying Chao , Hui Wang , Hong Pan , Quangang Yang , Xiumin Cui , Yanhong Lou , Yuping Zhuge

Lead (Pb), a highly toxic metal ion, is detrimental to plants and humans. Existing botanical techniques for Pb-contaminated soil remediation are limited in their efficiency. Here, we investigated the use of the fungus Trichoderma asperellum Samuels, Lieckf & Nirenberg SD-5, which we identified previously as being Pb-resistant, for phytoremediation and for its effects on plant growth, Pb adsorption, and physiological responses in perennial ryegrass (Lolium perenne L. ‘Lark’). We set up four soil treatments: CK (uncontaminated by Pb), T1 (1000 mg kg–1 Pb), T2 (1:9 ratio of sawdust to T1), and T3 (T2 inoculated with T. asperellum SD-5). A pot experiment revealed that the addition of the Pb-resistant microorganism promoted growth and increased biomass in ryegrass under Pb stress, in addition to significantly enhancing photosynthesis by increasing the leaf chlorophyll content and improving the total protein content and expression of the pAPX, POD, SOD, and GPX genes, evidence of an improved antioxidant system and the alleviation of Pb stress. We demonstrated that Pb-resistant microorganisms can enhance Pb extraction from the soil, thus improving remediation. Mitigation mechanisms operating at the physiological and gene expression levels were also determined, providing a scientific basis for the role of combined plant–microorganism methods in remediating Pb-contaminated soil.



中文翻译:

耐Pb真菌曲霉木霉SD-5减轻多年生黑麦草中的铅毒性和植物刺激作用

铅(Pb)是一种剧毒的金属离子,对植物和人类有害。现有的用于修复被Pb污染的土壤的植物技术的效率受到限制。在这里,我们调查了真菌Triperderma asperellum Samuels,Lieckf和Nirenberg SD-5的用途,我们先前确定其对Pb具有抗性,可用于植物修复及其对多年生黑麦草中植物生长,Pb吸附和生理反应的影响(黑麦草Perenne L. 我们建立了四种土壤处理方法:CK(未被Pb污染),T1(1000 mg kg –1 Pb),T2(锯末与T1的比例为9:9)和T3(接种了曲霉的T2)SD-5)。通过盆栽试验表明,在添加铅抗性微生物的促进生长和通过增加叶片的叶绿素含量和改善的总蛋白含量和表达的Pb胁迫下黑麦草增加的生物量,除了显著提高光合作用pAPXPODSODGPX基因,抗氧化剂系统改善和铅胁迫减轻的证据。我们证明了抗Pb微生物可以增强从土壤中Pb的提取,从而改善治理。还确定了在生理和基因表达水平上起作用的缓解机制,为组合的植物-微生物方法在修复受铅污染的土壤中的作用提供了科学依据。

更新日期:2020-12-01
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