5‐Aza‐2'‐deoxycytidine (5‐Aza‐dC), an inhibitor of DNA methyltransferases, is an effective treatment for various cancers and has improved the development rate of cloned embryos. Previous studies have reported the effect of 5‐Aza‐dC on fibroblasts; however, the mechanism whereby 5‐Aza‐dC affects sika deer granulosa cells and hormone secretion is presently unknown. Here, we showed that the cell cycle after treatment with different doses of 5‐Aza‐dC was significantly altered. The number of cells in the S phase was significantly increased in response to a concentration of 0.1 μM 5‐Aza‐dC. The rate of apoptosis was increased when cells were treated with 0.1 μM and 5 μM 5‐Aza‐dC. We showed that the protein level of H3K9me2 was significantly decreased in response to 5‐Aza‐dC. The activity levels of DNA methyltransferase were reduced by a moderate dose of 5‐Aza‐dC. Furthermore, the secretion of E₂ and P₄ was influenced by different doses of 5‐Aza‐dC. Our study suggested that 5‐Aza‐dC affected hormone secretion in sika deer granulosa cells through cell development and epigenetic regulation. The findings of this study lay the foundation for further epigenetic studies in sika deer.

中文翻译：

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5-氮杂-2'-脱氧胞苷对梅花鹿卵巢颗粒细胞激素分泌和表观遗传调控的影响

DNA甲基转移酶抑制剂5-Aza-2'-脱氧胞苷（5-Aza-dC）是一种有效的治疗多种癌症的方法，并提高了克隆胚胎的发育速度。先前的研究已经报道了5-Aza-dC对成纤维细胞的影响。然而，目前尚不清楚5-Aza-dC影响梅花鹿颗粒细胞和激素分泌的机制。在这里，我们显示了不同剂量的5-Aza-dC处理后的细胞周期发生了显着变化。浓度为0.1μM5-Aza-dC时，S期细胞数明显增加。当细胞用0.1μM和5μM5-Aza-dC处理时，凋亡率增加。我们显示，响应5-Aza-dC，H3K9me2的蛋白质水平显着降低。适量的5-Aza-dC降低了DNA甲基转移酶的活性水平。此外，E的分泌₂和P ₄受不同剂量的5-Aza-dC影响。我们的研究表明5-Aza-dC通过细胞发育和表观遗传调控影响了梅花鹿颗粒细胞中的激素分泌。这项研究的发现为梅花鹿的进一步表观遗传学研究奠定了基础。