Bemisia tabaci is important in agriculture worldwide, mainly because it is a vector of numerous plant viruses, probably the most important of which are members of the genus Begomovirus. Dozens of begomoviruses have been reported to infect tomato plants in Brazil, although tomato severe rugose virus (ToSRV) predominates in tomato crops. ToSRV, found so far only in Brazil, is efficiently transmitted by B. tabaci MEAM1. However, no studies have assessed the occurrence of vertical and horizontal transmission of the virus in the insect, which may have epidemiological consequences affecting disease management. This study evaluated the possibility of transmission of ToSRV between whiteflies during copulation and transovarial transmission from viruliferous females of B. tabaci MEAM1 to their progeny. Transmission of ToSRV did not occur during mating between males and females of B. tabaci MEAM1. Aviruliferous males and females confined with viruliferous insects of the opposite sex were also unable to transmit the virus to tomato plants. ToSRV was detected, by PCR, in the ovaries of viruliferous females of B. tabaci MEAM1 but not in eggs, nymphs, or adults of the progeny of viruliferous females. Adult progeny of viruliferous females also did not transmit ToSRV to tomato plants. Together, the results indicate that vertical and horizontal transmission of ToSRV by B. tabaci MEAM1 is unlikely. Sustainable management of the tomato golden mosaic disease caused by ToSRV should continue to focus on using resistant varieties, managing sources of inoculum around tomato fields, and rational chemical control of the vector.

中文翻译：

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烟粉虱MEAM1没有水平或垂直传播番茄严重皱纹病毒的证据

烟粉虱在世界范围内的农业中都很重要，主要是因为它是多种植物病毒的载体，其中最重要的可能是双歧杆菌属的成员。据报道，尽管番茄严重的皱纹病毒（ToSRV）在番茄作物中占主导地位，但数十种begomoviruses会感染巴西的番茄植物。迄今为止仅在巴西发现的ToSRV由烟粉虱MEAM1有效地传播。但是，尚无研究评估该病毒在昆虫中垂直和水平传播的发生，这可能会影响疾病管理的流行病学后果。这项研究评估了交配期间粉虱之间ToSRV传播的可能性以及来自有毒女性的经卵巢传播的可能性。烟粉虱MEAM1到它们的后代。烟粉虱MEAM1的雌雄交配过程中未发生ToSRV的传播。受异性有毒昆虫限制的无毒雄性和雌性也无法将病毒传播给番茄植物。通过PCR，在烟粉虱MEAM1的有毒女性卵巢中检测到ToSRV，但在有毒女性后代的卵，若虫或成虫中未检测到ToSRV。有毒雌性的成年后代也没有将ToSRV传播给番茄植物。在一起，结果表明烟粉虱的ToSRV的垂直和水平传播。MEAM1不太可能。由ToSRV引起的番茄金色花叶病的可持续管理应继续集中在使用抗性品种，管理番茄田周围接种物的来源以及对载体进行合理的化学控制上。