Abstract Blueberry (Vaccinium spp., Vc) plants are highly susceptible to Fe deficiency stress. An understanding of the mechanisms involved in Fe as well as other mineral ions uptake and transportation is critical for the improvement of mineral ions use efficiency in the field practices. The functional roles of the VcZIPs in mineral ions uptake and transportation were not well understood. To elucidate the functions of ZIP transporter family that is involved in the uptake and transport of metal elements in blueberry, VcZIPs such as VcZIP1, VcZIP2, VcZIP6, VcZIP7, VcZIP8 and VcZIP11 were cloned and identified from blueberry genome. The results of functional complementarity and heterologous expression of yeast mutant verified that most of VcZIPs had the ability to transport Fe and Zn, and VcZIP1 and VcZIP7 could mediate the transport of Cd in yeast. Furthermore, higher chlorophyll contents and bigger crown diameter sizes were observed in VcZIP6, VcZIP7 and VcZIP8 over-expressed Arabidopsis thaliana at Fe deficient treatments. In this study, VcZIP1, VcZIP2, VcZIP6–VcZIP8 and VcZIP11 were Fe-deficient responsive genes in blueberry. VcZIP6–VcZIP8 participated in the uptake of Fe and likely contributed to Fe uptake and transportation in plants. VcZIP1, VcZIP7, VcZIP8 and VcZIP11 were involved transportation Zn in blueberry. Comparatively, higher Zn-transport abilities were observed in VcZIP1 and VcZIP7.

中文翻译：

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蓝莓ZIP转运蛋白的克隆及功能分析

摘要 蓝莓 (Vaccinium spp., Vc) 植物对缺铁胁迫高度敏感。了解 Fe 以及其他矿物离子吸收和运输的机制对于提高现场实践中矿物离子的利用效率至关重要。VcZIPs 在矿物质离子吸收和运输中的功能作用尚不清楚。为了阐明参与蓝莓中金属元素摄取和转运的 ZIP 转运蛋白家族的功能，从蓝莓基因组中克隆并鉴定了 VcZIP，如 VcZIP1、VcZIP2、VcZIP6、VcZIP7、VcZIP8 和 VcZIP11。酵母突变体的功能互补和异源表达结果证实，大部分VcZIPs具有转运Fe和Zn的能力，VcZIP1和VcZIP7可以介导酵母中Cd的转运。此外，在 VcZIP6、VcZIP7 和 VcZIP8 过表达的拟南芥中在缺铁处理中观察到更高的叶绿素含量和更大的冠径。在本研究中，VcZIP1、VcZIP2、VcZIP6–VcZIP8 和 VcZIP11 是蓝莓中缺铁的响应基因。VcZIP6–VcZIP8 参与了铁的吸收，并可能有助于植物中铁的吸收和运输。VcZIP1、VcZIP7、VcZIP8和VcZIP11参与蓝莓中Zn的转运。相比之下，在 VcZIP1 和 VcZIP7 中观察到更高的锌转运能力。VcZIP6–VcZIP8 参与了铁的吸收，并可能有助于植物中铁的吸收和运输。VcZIP1、VcZIP7、VcZIP8和VcZIP11参与蓝莓中Zn的转运。相比之下，在 VcZIP1 和 VcZIP7 中观察到更高的锌转运能力。VcZIP6–VcZIP8 参与了铁的吸收，并可能有助于植物中铁的吸收和运输。VcZIP1、VcZIP7、VcZIP8和VcZIP11参与蓝莓中Zn的转运。相比之下，在 VcZIP1 和 VcZIP7 中观察到更高的锌转运能力。