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Transcriptomic analysis revealed distinctive modulations of arbuscular mycorrhizal fungi inoculation in halophyte Suaeda salsa under moderate salt conditions
Environmental and Experimental Botany ( IF 5.7 ) Pub Date : 2021-03-01 , DOI: 10.1016/j.envexpbot.2020.104337
Fengwei Diao , Zhenhua Dang , Xi Cui , Jing Xu , Bingbing Jia , Shengli Ding , Zhechao Zhang , Wei Guo

Abstract As the area of soil salinization increases, the ecological niche of halophytes is important given their high salt tolerance. The physiological response of mycorrhizal halophytes is not completely consistent with that of mycorrhizal glycophytes under salt conditions. However, less information is known about the regulation of genes included by arbuscular mycorrhizal (AM) fungi in halophytes. A greenhouse pot experiment with inoculation (Funneliformis mosseae) treatments was performed to research the physiological and transcriptomic responses in the halophyte Suaeda salsa under 100 mM NaCl. The results revealed that AM symbiosis significantly increased shoot biomass, shoot height and shoot Na+ accumulation. AM fungi decreased the Na+ concentration and soluble sugar concentration in the shoots of S. salsa. Transcriptomic analysis revealed 1316 and 424 differentially expressed genes (DEGs) in shoots and roots, respectively. DEGs in shoots were involved in carbohydrate and energy metabolism; photosynthesis; amino acid metabolism; cofactors and vitamins metabolism, such as glyoxylate and dicarboxylate metabolism; carbon fixation in photosynthetic organisms; and vitamin B6 metabolism. Interestingly, the expression of DEGs related to photosynthesis was downregulated by AM fungi in S. salsa under 100 mM NaCl. DEGs of roots were enriched in starch and sucrose metabolism; and auxin transmembrane transporters. These distinctive modulations of AM fungi in genes and physiological parameters are potentially associated with the growth of halophytes under moderate salt conditions. This study will improve our understanding of the effect of AM fungi in halophytes under moderate salt conditions and suggests that the phytoextractive strategy of halophytes combined with AM fungi hold significant ecological potential in salinized ecosystems.

中文翻译:

转录组学分析揭示了在中等盐度条件下对盐生植物 Suaeda salsa 接种丛枝菌根真菌的独特调节

摘要 随着土壤盐渍化面积的增加,盐生植物具有较高的耐盐性,其生态位非常重要。盐生植物菌根的生理反应与盐生植物菌根的生理反应并不完全一致。然而,关于盐生植物中丛枝菌根 (AM) 真菌所含基因调控的信息知之甚少。进行了接种 (Funneliformis mosseae) 处理的温室盆栽实验,以研究盐生植物 Suaeda salsa 在 100 mM NaCl 下的生理和转录组反应。结果表明,AM共生显着增加了枝条生物量、枝条高度和枝条Na+的积累。AM 真菌降低了 S. salsa 枝条中的 Na+ 浓度和可溶性糖浓度。转录组学分析显示,芽和根中分别有 1316 和 424 个差异表达基因(DEG)。枝条中的DEGs参与碳水化合物和能量代谢;光合作用; 氨基酸代谢;辅助因子和维生素代谢,如乙醛酸和二羧酸代谢;光合生物中的碳固定;和维生素 B6 代谢。有趣的是,在 100 mM NaCl 下,S. salsa 中的 AM 真菌下调了与光合作用相关的 DEG 的表达。根的DEGs富含淀粉和蔗糖代谢;和生长素跨膜转运蛋白。AM 真菌在基因和生理参数中的这些独特调节可能与中等盐度条件下盐生植物的生长有关。
更新日期:2021-03-01
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