Radioimmunoassay (RIA) is an extremely specific and a highly sensitive type of immunoassay, but the long incubation time and generation of radioactive wastes limit the use of RIA. To complement these disadvantages of RIA, we suggest an advanced type of RIA based on a lab-on-a-chip (LOC) platform: μ-RIA. We designed a microfluidic chip for RIA and optimized the procedures of μ-RIA analysis, including surface modification, immunoreaction time, and washing. Based on the optimized conditions, we conducted a radioimmunoassay on the μ-RIA platform using a commercial RIA kit. With the μ-RIA, 5 min are adequate for analysis. The amount of reagent consumption is significantly reduced compared with conventional RIA. The standard curve with R² = 0.9951 shows that we can quantitatively evaluate the amount of antigen present in unknown samples. We show the applicability of μ-RIA for the analysis of biomolecules and the potential of μ-RIA to be a novel platform for high-throughput analysis.

放射免疫测定 (RIA) 是一种极其特异且高度灵敏的免疫测定类型，但较长的孵育时间和放射性废物的产生限制了 RIA 的使用。为了弥补 RIA 的这些缺点，我们建议使用一种基于芯片实验室 ​​(LOC) 平台的高级 RIA：μ-RIA。我们设计了一种用于 RIA 的微流控芯片，并优化了 μ-RIA 分析的程序，包括表面修饰、免疫反应时间和洗涤。基于优化的条件，我们使用商业 RIA 试剂盒在 μ-RIA 平台上进行了放射免疫测定。使用 μ-RIA，5 分钟足以进行分析。与传统 RIA 相比，试剂消耗量显着减少。R ²的标准曲线 = 0.9951 表明我们可以定量评估未知样品中存在的抗原量。我们展示了 μ-RIA 在生物分子分析中的适用性以及 μ-RIA 成为高通量分析新平台的潜力。