Abstract

Nanomaterials can exhibit a more pronounced genotoxic effect compared to the individual components contained in them, changing the structure of DNA and histone proteins, as well as the amount of these substances. We analyzed the impact of the Keplerate-type nanocluster iron–molybdenum polyoxometalate (POM) and POM degradation products in solutions on the content of nucleic acids, free nucleotides, and histone proteins in blood lymphocytes of Wistar rats. We revealed a reversible increase in the amount of DNA after a single injection of POM with a dose of 0.15 mg/100 g and no decrease in the histone content after 1, 7, and 30 injections. On the contrary, the products of degradation of the same POM dose changed the level of DNA, RNA, free nucleotides, total histone number, and the histone fractions. Preservation of the total number of lymphocytes during thirty-fold exposure to POM, in contrast to the effects of degradation products, confirms the less pronounced toxic effect of POM compared to the action of the POM component mixture.

中文翻译：

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铁钼多金属氧酸盐和纳米颗粒成分的混合物对大鼠淋巴细胞中核酸和组蛋白的含量的影响

摘要

与纳米材料中包含的单个成分相比，纳米材料可以表现出更明显的遗传毒性作用，从而改变DNA和组蛋白的结构以及这些物质的数量。我们分析了Keplerate型纳米簇多金属氧钼铁（POM）和POM降解产物对Wistar大鼠血液淋巴细胞中核酸，游离核苷酸和组蛋白的含量的影响。我们发现，单次注射0.15 mg / 100 g的POM后，DNA量可逆增加，而在注射1、7和30次后组蛋白含量没有降低。相反，相同POM剂量的降解产物改变了DNA，RNA，游离核苷酸，总组蛋白数和组蛋白级分的水平。