Applied Biochemistry and Microbiology ( IF 0.8 ) Pub Date : 2020-12-01 , DOI: 10.1134/s0003683820090069 S. V. Nikulin , M. P. Raigorodskaya , D. A. Sakharov
Caco-2 cells are traditionally used to construct in vitro models of the intestinal barrier. One characteristic of the mature intestine is the presence of villi—connective tissue outgrowths covered with epithelial cells. It was recently shown that Caco-2 cells form structures resembling intestinal villi during prolonged cultivation. In this work, we showed via transcriptome analysis that the BMP and PDGF signaling cascades involved in the formation of villi in vivo are significantly altered during the differentiation of Caco-2 cells and, therefore, can participate in the formation of similar structures in vitro. In particular, we found a significant decrease in the expression of the BMP4, BMP7, and BMP8A genes in differentiated cells as compared to undifferentiated cells. We also first discovered periodic fluctuations in transepithelial resistance upon the differentiation of Caco-2 cells. The period of observed fluctuations indicates that they can occur as a result of cell proliferation during villus formation.
中文翻译:
转录组分析涉及肠绒毛形成的Caco-2细胞的信号通路。
传统上,Caco-2细胞用于构建肠道屏障的体外模型。成熟肠的一个特征是绒毛的存在-上皮细胞覆盖着结缔组织。最近显示,在长期培养期间,Caco-2细胞形成类似于肠绒毛的结构。在这项工作中,我们通过转录组分析显示,参与体内绒毛形成的BMP和PDGF信号级联在Caco-2细胞分化过程中发生了显着改变,因此可以参与体外相似结构的形成。特别是,我们发现BMP4,BMP7和BMP8A的表达显着下降与未分化细胞比较 我们还首次发现了Caco-2细胞分化后跨上皮抵抗的周期性波动。观察到的波动时期表明,它们可能是由于绒毛形成过程中细胞增殖而发生的。