The fungus, Beauveria bassiana (Bals.) Vuil., is one of the most important entomopathogenic fungi (EPFs). Recently, its new role was discovered in nature, to be an endophyte in plants. It has been reported as an endophytic fungus in many monocotyledonous and dicotyledonous plants. The study was conducted to evaluate the ability of the fungus, B. bassiana, to colonize and persist in cucumber plants under laboratory conditions and to detect its systemic growth inside the plant tissues in addition to pathogenicity in the plant. The isolate, B195, of the fungus, B. bassiana, was used. Five different inoculation methods were followed: seed dusting, seed immersion, soil drench, seedling drench, and foliar spray. The fungus, B. bassiana, could persist inside different cucumber tissues up to 90 days from inoculation. Soil drench provided the highest recovery rates, while foliar spray gave the lowest rates. Colonization rates reached 94.44 and 73.68% for stem and 68.26 and 37.79% for root, 30 and 90 days post soil drench, respectively, while in foliar spray, it reached 33.51 and 16.45%, after 30 and 90 days post-treatment, for the stem and 9.45 and 0% for the root, respectively. No negative effects were observed in inoculated plants or on fungal pathogenicity. Results showed for the first time the ability of the fungus, B. bassiana, isolate B195, to artificially colonize and survive in different parts of cucumber plants under laboratory conditions by different inoculation methods and to grow systemically in plant tissues. This study is considered a preliminary study to the utilization of the fungus, B. bassiana, as an endophyte in cucumber plants to reduce the density of insect pests.

中文翻译：

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在黄瓜植物中内生真菌真菌致病性球孢白僵菌（Balveria bassiana（Bal。）Vuil。）的建立

真菌球孢白僵菌（Balveria bassiana（Bal。）Vuil。）是最重要的致病性真菌（EPF）之一。最近，它的新作用在自然界被发现，是植物中的内生菌。据报道在许多单子叶和双子叶植物中作为内生真菌。这项研究的目的是在实验室条件下评估真菌B. bassiana在黄瓜植株中的定殖和持久能力，以及检测其在植物组织内的全身生长以及致病性的能力。使用了真菌B. bassiana的分离物B195。遵循了五种不同的接种方法：种子除尘，种子浸入，土壤浸湿，幼苗浸湿和叶面喷雾。真菌B. bassiana可以在接种后长达90天的时间内保留在不同的黄瓜组织中。土壤淋水提供最高的恢复率，而叶面喷雾提供最低的恢复率。在土壤浸湿后30天和90天，茎的定植率分别达到94.44％和73.68％，根部定植分别为68.26％和37.79％，而叶面喷洒处理后30天和90天分别达到33.51％和16.45％。茎，根分别为9.45和0％。在接种的植物或真菌致病性中未观察到负面影响。结果首次显示了真菌B. bassiana，分离物B195在实验室条件下通过不同接种方法在黄瓜植物的不同部位人工定居和存活并在植物组织中系统生长的能力。这项研究被认为是对真菌B. bassiana的利用的初步研究，