Abstract Microorganisms play an important role in the circulation of phosphorus (P) in the sediment of coastal wetland ecosystems. In this study, solution 31P nuclear magnetic resonance (NMR) was used to determine different forms of P in the sediments of four different seagrass meadows and a bare tidal flat, while high-throughput 16S and ITS rRNA gene sequencing was used to determine the microbial community composition. The solution 31P-NMR spectra revealed six forms of the P compounds detected by the NaOH-EDTA extraction of sediments, where Ortho-P was the most dominant P compound, followed by Mono-P. The Po compounds were more varied in the seagrass meadow sediments and more abundant compared to the bare tidal flat. Bacterial communities in the sediments collected from E. acoroides and fungal communities in the bare tidal flat were relatively different from those at the other sites. The relative abundance of P-cycling-related fungi belonging to the phylum Ascomycota was 26.20% and was much higher than that of bacteria (only 0.29%) belonging to the class Bacilli. Mono-P was the major factor determining the distribution of P-cycling-related fungi and negatively correlated with the relative abundance of Aspergillus and Trichoderma. We believe that fungi can affect P forms in the sediment of seagrass meadows more than bacteria.

中文翻译：

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主要受真菌而非细菌影响的海草草甸沉积物中的磷形态：基于 31P-NMR 和高通量测序的初步研究

摘要 微生物在滨海湿地生态系统沉积物中磷（P）的循环中起重要作用。本研究采用溶液 31P 核磁共振 (NMR) 测定了四种不同海草草甸和一个裸滩沉积物中不同形式的 P，同时采用高通量 16S 和 ITS rRNA 基因测序来测定微生物社区组成。溶液 31P-NMR 光谱揭示了通过 NaOH-EDTA 提取沉积物检测到的六种形式的 P 化合物，其中 Ortho-P 是最主要的 P 化合物，其次是 Mono-P。与裸滩涂相比，海草草甸沉积物中的 Po 化合物更多样化，更丰富。从 E. 收集的沉积物中的细菌群落。裸露滩涂中的菌群和真菌群落与其他地点的相对不同。属于子囊菌门的 P-循环相关真菌的相对丰度为 26.20%，远高于属于杆菌类的细菌（仅 0.29%）。Mono-P是决定P-循环相关真菌分布的主要因素，与Aspergillus和Trichoderma的相对丰度呈负相关。我们相信真菌比细菌更能影响海草草地沉积物中的磷形态。Mono-P是决定P-循环相关真菌分布的主要因素，与Aspergillus和Trichoderma的相对丰度呈负相关。我们相信真菌比细菌更能影响海草草地沉积物中的磷形式。Mono-P是决定P-循环相关真菌分布的主要因素，与Aspergillus和Trichoderma的相对丰度呈负相关。我们相信真菌比细菌更能影响海草草地沉积物中的磷形态。