In eukaryotic cells, pre-mRNA splicing is catalyzed by the spliceosome, a highly dynamic molecular machinery that undergoes dramatic conformational and compositional rearrangements throughout the splicing cycle. These crucial rearrangements are largely driven by eight DExD/H-box RNA helicases. Interestingly, the four helicases participating in the late stages of splicing are all DEAH-box helicases that share structural similarities. This review aims to provide an overview of the structure and function of these DEAH-box helicases, including new information provided by recent cryo-electron microscopy structures of the spliceosomal complexes.

中文翻译：

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Pre-mRNA 剪接中的 DEAH-Box RNA 解旋酶

在真核细胞中，前 mRNA 剪接由剪接体催化，剪接体是一种高度动态的分子机器，在整个剪接周期中经历剧烈的构象和组成重排。这些重要的重排主要是由八个 DExD/H-box RNA 解旋酶驱动的。有趣的是，参与剪接后期的四个解旋酶都是具有结构相似性的DEAH盒解旋酶。本综述旨在概述这些 DEAH-box 解旋酶的结构和功能，包括最近剪接体复合物的冷冻电子显微镜结构提供的新信息。